258例血清中抗-HCV阳性联合HCV-RNA定量检测结果分析  

Result analysis of serum anti-HCV positive combined with HCV-RNA quantitative detection of 258 cases

作　　者：庞丽娜[1] 薛金方[1] 

机构地区：[1]中山大学附属第五医院检验科,519000

出　　处：《中国实用医药》2014年第29期34-35,共2页China Practical Medicine

摘　　要：目的探讨丙型肝炎病毒抗体(抗-HCV)和丙型肝炎病毒核糖核酸(HCV-RNA)定量联合检测结果对丙型肝炎确诊的临床意义。方法对258例ELISA法检测的血清抗-HCV阳性丙型肝炎(HC)患者,同时采用PCR-荧光探针法检测其HCV-RNA含量。结果 258例抗-HCV阳性丙型肝炎患者中HCV-RNA阴性92例,占35.66%;17例HCV-RNA弱阳性,占6.59%;HCV-RNA阳性149例,即57.75%的HC患者存在病毒血症。结论 ELISA法联合检测抗-HCV和HCV-RNA有助于丙型肝炎确诊病毒血症以及监控HCV的感染,并及时进行抗病毒治疗。Objective To investigate the clinical significance of hepatitis C virus antibody （anti-HCV） combined with hepatitis C virus ribonucleic acid （HCV-RNA） quantitative detection in the diagnosis of hepatitis C （HC）. Methods A total of 258 cases of HC with anti-HCV positive were detected by ELISA method and PCR- fluorescence probe for the HCV-RNA content. Results Among the 258 cases with anti-HCV positive, there were 92 cases of HCV-RNA negative as 35.66%, 17 cases of HCV-RNA weakly positive as 6.59%, and 149 cases of HCV-RNA positive. Thus, there were 57.75% of the HC patients with viremia. Conclusion ELISA method in combined detection of anti-HCV and HCV-RNA helps to diagnose viremia of HC, monitor HCV infection and conduct timely antiviral treatment.

关 键 词：丙型肝炎病毒抗体 丙型肝炎病毒核糖核酸 丙型肝炎 PCR-荧光探针 

分 类 号：R512.63[医药卫生—内科学]