切口痛大鼠十二指肠胃动素水平的变化  

Changes in levels of motilin in duodenum in a rat model of incisional pain

作　　者：赵军[1] 张瑜[2] 高宝柱[1] 郑宝森[3] 曹君利[4] 

机构地区：[1]天津市第三中心医院麻醉科,300170 [2]天津医科大学附属肿瘤医院麻醉科 [3]天津医科大学第二医院疼痛治疗中心 [4]徐州医学院江苏省麻醉与镇痛应用技术重点实验室

出　　处：《中华麻醉学杂志》2014年第9期1089-1091,共3页Chinese Journal of Anesthesiology

基　　金：天津市卫生局科技基金（2010KZ19）

摘　　要：目的 评价切口痛大鼠十二指肠胃动素水平的变化.方法 SPF级健康雄性SD大鼠84只,6～8周龄,体重180 ～ 220 g,采用随机数字表法,将其分为2组（n=42）：对照组（C组）和切口痛组（P组）.行右足跖肌切口以制备切口痛模型.2组取6只大鼠,于术前24 h（T0）、术后1 h（T1）、6h（T2）、24 h（T3）、48 h（T4）、72 h（T5）时测定机械缩足反应阈（MWT）与热缩足反应潜伏期（TWL）,并于上述各时点分别取6只大鼠,处死,取十二指肠粘膜组织,采用ELISA法测定胃动素水平.P组各时点十二指肠胃动素含量与痛阈之间进行Pearson相关分析.结果 与C组比较,P组T1-4时MWT降低,TWL缩短,十二指肠胃动素含量升高（P＜0.05）,T0.5时差异无统计学意义（P＞0.05）.P组十二指肠胃动素水平与MWT和TWL均呈负相关,r分别为-0.8 910和-0.8 463（P＜0.01）.结论 切口痛可促进大鼠十二指肠胃动素分泌.Objective To evaluate the changes in the levels of motilin in the duodenum in a rat model of incisional pain.Methods Eighty-four healthy male Sprague-Dawley rats,aged 6-8 months,weighing 180-220 g,were randomized into 2 groups （n =42 each） using a random number table：control group （group C） and incisional pain group （group P）.The animals were anesthetized with sevoflurane.In group P,a 1 cm long incision was made in the plantar surface of right hindpaw.Six rats were chosen from each group and mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 24 h before operation （T0） and 1,6,24,48 and 72 h after operation （T1-5）.Six rats were chosen from each group at T0-5 and sacrificed and the duodenal mucosal tissue was prepared for measurement of motilin levels by ELISA.Pearson linear correlate analysis was performed between the motilin level and pain threshold at each time point in group P.Results Compared with group C,MWT was significantly decreased,TWL was shortened,and motilin levels were significantly increased at T1-4,and no significant change was found at T0 and T5 in group P.The motilin levels were negatively correlated with MWT （r =-0.8 910） and TWL （r =-0.8 463） in group P.Conclusion Incisional pain can promote the secretion of motilin in the duodenum.

关 键 词：疼痛 手术后 促胃动素 十二指肠 

分 类 号：R614[医药卫生—麻醉学]