脊髓组蛋白去乙酰化酶在大鼠神经病理性痛维持中的作用  

Role of histone deacetylase in spinal cord in maintenance of neuropathic pain in rats

作　　者：欧阳碧函[1] 翁莹琪[1] 陈旦[1] 郭曲练[1] 

机构地区：[1]中南大学湘雅医院麻醉科,长沙市410008

出　　处：《中华麻醉学杂志》2014年第9期1095-1097,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81171053）

摘　　要：目的 评价脊髓组蛋白去乙酰化酶在大鼠神经病理性痛维持中的作用.方法 健康成年雄性SD大鼠27只,体重230 ～ 270 g,采用随机数字表法,将其分为3组（n=9）：假手术组（S组）、神经病理性痛组（NP组）、神经病理性痛＋组蛋白去乙酰化酶抑制剂曲古霉素A组（T组）.采用坐骨神经慢性压迫性损伤（CCI）法建立大鼠神经病理性痛模型.于术后7d,分别鞘内注射5％DMSO溶液（S组）、5％ DMSO溶液（NP组）、曲古霉素A（T组）10 μg各10 μL,1次/d,连续3d.于术前1d、术后3、7、10、14和21 d（T0-5）时测定术侧后足机械缩足反应阈（MWT）和热缩足反应潜伏期（TWL）.结果 与S组比较,NP组和T组T2-5时MWT降低,TWL缩短（P＜0.05）;与NP组比较,T组T3.4时MWT升高,TWL延长（P＜0.05）.结论 脊髓组蛋白去乙酰化酶参与大鼠神经病理性痛的维持.Objective To evaluate the role of histone deacetylase in the spinal cord in the maintenance of neuropathic pain （NP） in rats.Methods Twenty-seven male Sprague-Dawley rats,weighing 230-270 g,were randomly divided into 3 groups （n =9 each） using a random number table：sham operation group （group S）,group NP,and NP ＋ intrathecal Trichostatin A （TSA） group （group T）.NP was induced by chronic constrictive injury.At 7 days after operation,5% DMSO,5%DMSO and TSA 10 μg （10 μl） were injected intrathecally once a day for 3 consecutive days in S,NP and T groups,respectively.Mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 1 day before operation and 3,7,10,14 and 21 days after operation （T0-5）.Results Compared with group S,MWT was significantly decreased and TWL was shortened at T2-5 in NP and T groups.Compared with group NP,MWT was significantly increased and TWL was prolonged at T3,4 in group T.Conclusion Histone deacetylase in the spinal cord is involved in the maintenance of neuropathic pain in rats.

关 键 词：组蛋白脱乙酰基酶类 神经痛 

分 类 号：R402[医药卫生—临床医学]