瑞芬太尼后处理对大鼠心肌缺血再灌注损伤时水通道蛋白1表达的影响  

Effects of remifentanil post-conditioning on aquaporin-1 expression during myocardial ischemia-reperfusion injury in rats

作　　者：赖忠盟[1] 郑宏[1] 陈文华[1] 张良成[1] 林鹏焘[1] 

机构地区：[1]福建医科大学附属协和医院麻醉科,福州350001

出　　处：《中华麻醉学杂志》2014年第9期1128-1130,共3页Chinese Journal of Anesthesiology

基　　金：福建省教育厅科技项目（JA10153）

摘　　要：目的 评价瑞芬太尼后处理对大鼠心肌缺血再灌注损伤时水通道蛋白1（AQP-1）表达的影响.方法 健康雄性SD大鼠24只,体重250 ～ 300 g,采用随机数字表法将其分为3组（n=8）：假手术组（S组）、缺血再灌注组（I/R组）和瑞芬太尼后处理组（RP组）.采用结扎左冠状动脉前降支45 min再灌注24 h的方法建立心肌缺血再灌注损伤模型.RP组于再灌注前10 min开始静脉输注瑞芬太尼10 μg·kg-·min-1,经10 min输注完毕,S组和I/R组给予等容量生理盐水.于再灌注结束时处死大鼠,取左心室组织,光镜下观察心肌组织病理学结果,采用实时荧光定量PCR法测定缺血区心肌组织AQP-1mRNA表达,采用Western blot法测定缺血区心肌组织AQP-1蛋白表达,计算心肌含水率.结果 与S组比较,其余2组缺血区心肌组织含水率增加,I/R组心肌组织AQP-1 mRNA和蛋白表达上调（P＜0.05）,RP组心肌组织AQP-1 mRNA和蛋白表达比较差异无统计学意义（P＞0.05）;与I/R组比较,RP组心肌组织含水率减少,心肌组织AQP-1 mRNA和蛋白表达下调（P＜0.05）.结论 瑞芬太尼后处理可能通过下调心肌组织AQP-1表达减轻大鼠心肌缺血再灌注损伤.Objective To evaluate the effects of remifentanil post-conditioning on aquaporin-1 （AQP-1） expression during myocardial ischemia-reperfusion （I/R） injury in rats.Methods Twenty-four male.SpragueDawley rats,weighing 250-300 g,were randomly divided into 3 groups （n =8 each） using a random number table：sham operation group （group S）,group I/R,and remifentanil post-conditioning group （group RP）.Myocardial I/R was induced by 45 min occlusion of left anterior descending branch of coronary artery followed by 24 h reperfusion.Remifentanil 10 μg· kg-1· min-1 was infused over 10 min starting from 10 min before reperfusion in group RP,while the equal volume of normal saline was given instead in S and I/R groups.At the end of reperfusion,all the rats were sacrificed and their myocardial specimens from left ventricles were obtained for microscopic examination of thepathological changes and for determination of AQP-1 mRNA （using real-time fluorescent quantitative PC R） and AQP-1 protein （by Western blot） expression in the ischemic area and myocardial water content.Results Compared with S group,myocardial water content was significantly increased in the other two groups,AQP-1 mRNA and protein expression was up-regulated in group I/R,and no significant change was found in AQP-1 mRNA and protein expression in RP group.Compared with I/R group,myocardial water content was significantly reduced,and AQP-1 mRNA and protein expression was down-regulated in RP group.Conclusion Remifentanil post-conditioning reduces myocardial I/R injury possibly through down-regulating AQP-1 expression in myocardial tissues of rats.

关 键 词：哌啶类 心肌再灌注损伤 水通道蛋白质1 

分 类 号：R614[医药卫生—麻醉学]