三种生长抑制剂对嗜盐海洋着色菌光合色素合成的影响  

Effects of Growth Inhibitors on Photopigment Biosynthesis in Halophilic Marichromatium sp.283-1

作　　者：贾雅琼[1] 洪璇[1,2] 姚鹏[1] 赵春贵[1] 杨素萍[1] 

机构地区：[1]华侨大学生物工程与技术系,福建厦门361021 [2]厦门医学高等专科学校,福建厦门361021

出　　处：《山西大学学报（自然科学版）》2014年第4期637-642,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家海洋公益性行业科研史项(201505026);国家自然科学基金(No.31070054;No.31270106);福建省自然科学基金(No.2012J01136);福建省教育厅科技项目(No.JB11275)

摘　　要：采用吸收光谱法、薄层层析法（TLC）和图像灰度分析法,系统研究了光照厌氧条件下甘氨酸（Gly）、亚硝氮（NO-2-N）和二苯胺（DPA）对海洋着色菌（Marichromatiumsp.）283-1生长和光合色素合成的影响。结果表明：0-15mmol/L Gly对菌体生长无明显影响,低于7mmol/L时,随Gly浓度升高,菌体积累的类胡萝卜素（Car）和细菌叶绿素（BChl）含量以及Car途径中末端产物奥氏酮（Okenone）相对含量升高,高于7mmol/L时色素变化规律相反。低浓度（7mmol/L）NO-2-N对菌体生长、Car和BChl的合成都有促进作用,高浓度明显抑制菌体生长、Car和BChl的合成,但Okenone相对含量则随NO-2-N浓度升高而呈上升趋势。DPA降低了菌体生长速率、最终生物量、Car和BChl合成量以及Okenone相对含量,而BChl相对含量则逐渐升高,与对照相比,菌体还积累了另外2种Car组分,且随DPA浓度的升高其相对含量也逐渐增加。由此可见,Gly、NO2^--N和DPA明显影响283-1菌株光合色素的合成,DPA能使菌体积累更多的Car组分。The effects of glycine,nitrite and diphenylamine on bacterial growth and photopigment biosynthesis of Marichromatiumsp.283-1anaerobically in the light were investigated.The results showed that 0-15mmol/L glycine had little effect on bacterial growth.The total amounts of Car and BChl as well as the relative content of Okenone were increased when glycine concentration was less than 7mmol/L,whereas were decreased when glycine concentration was more than 7 mmol/L.The bacterial growth and the total amounts of Car and BChl were promoted by low-concentration nitrite,while were obviously inhibited by high-concentration nitrite.The relative content of Okenone was increased with increasing nitrite concentration.The addition of Diphenylamine decreased the bacterial growth rate,final biomass,the total amounts of Car and BChl as well as the relative content of Okenone,while the relative content of BChl was gradually increased.Two additional Car components were detected in Diphenylamine treatment cells,and their relative contents were gradually increased with increasing Diphenylamine concentration.

关 键 词：海洋着色菌 光合色素 奥氏酮 

分 类 号：Q935[生物学—微生物学]