芩术四物汤水煎液中黄芩苷、黄芩素和汉黄芩素的含量测定  

Determination of baicalin,baicalein and wogonin in decoction of Qinzhu Siwutang

作　　者：袁海梅[1,2,3] 彭腾[3] 黄道秋[4] 韩平 郭廷东[1,2] 宋良玉 胡晓燕[1,2] 王丹 

机构地区：[1]南充市中心医院 [2]川北医学院第二临床医学院药学部,四川南充637000 [3]成都中医药大学药学院教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075 [4]重庆三峡中心医院医务科,重庆404000

出　　处：《中国医院药学杂志》2014年第21期1842-1844,共3页Chinese Journal of Hospital Pharmacy

基　　金：重庆市科委重点项目(编号:CSTC2011AC5141);四川省教育厅重点课题(编号:10ZA095)

摘　　要：目的:建立HPLC法同时测定芩术四物汤水煎液中黄芩苷、黄芩素和汉黄芩素含量测定的方法。方法:采用Kromasil ODS-C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水为流动相,梯度洗脱,柱温:35℃,流速:0.8 mL·min-1,检测波长273 nm。结果:黄芩苷、黄芩素和汉黄芩素线性范围良好,加样回收率分别为102.48%、102.19%和103.13%。结论:所用方法简单、快捷、重复性好、准确度高,可用于芩术四物汤水煎液的含量测定。OBJECTIVE To detenmne baiealin, baicalein and wogonin contents in Qinzhu Siwutang by HPLC. METHODS The separation was performed on an Krornasil ODS-C18 column （4. 6 mm×250 mm, 5μm） with gradient elution, the mobile phases consisted of acetonitrile and 0. 1 -1 phosphoric acid at a flow rate of 0. 8 mL. min^-1. The detective wavelength was 273 nm, the temperature was 35 ℃. RKS-＇ULIB To determine baicalin, baicalein and wogonin contents in Qinzhu Siwutang by RP-HPLC, baicalin, baicalein and wogonin showed good linearity （r2≥0. 999 3） in the ranges of tested concentration; the recovery rates （102. 48%, 102. 19% and 103. 13%） were satisfactory. CI）NCLUSION The method for determination of 3 flavonoids compounds in Qinzhu Siwutang, the meth- od can be used for rapid and convenient evaluation of the quality of Qinzhu Siwu^ang.

关 键 词：芩术四物汤 高效液相色谱法 黄芩苷 黄芩素 汉黄芩素 

分 类 号：R927[医药卫生—药学]