阿托伐他汀与雷帕霉素联合应用对彼此在小肠吸收的影响  

Study on the effects of rapamycin in combination with statins on the intestinal absorption in rats

作　　者：安小通[1] 孙成春[2] 毕玲玲[1] 贾暖[2] 董玉波[2] 

机构地区：[1]辽宁医学院,辽宁锦州1210012 [2]济南军区总医院药剂科,山东济南250031

出　　处：《中国医院药学杂志》2014年第21期1867-1869,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:通过离体和在体两种模型探讨阿托伐他汀与雷帕霉素联合应用在大鼠小肠被吸收的状况。方法:利用高相液相色谱仪建立同时测定肠灌流液中阿托伐他汀和雷帕霉素浓度的分析方法;建立离体与在体两种模型研究药物在小肠吸收的情况。离体模型采用外翻小肠囊法收集肠透膜液样本,在体模型采用单向肠灌流模型采集肠液;最后,利用高效液相色谱法测定肠灌流液中雷帕霉素及阿托伐他汀的浓度。结果:阿托伐他汀与雷帕霉素联合应用后,在小肠的吸收量明显高于单药组。结论:阿托伐他汀与雷帕霉素联合应用对两种药物在肠道的吸收都有明显的增强作用。OBJECTIVE To explore the effects of rapamycin in combination with atorvastatin on the intestinal absorption by in vitro and in situ models in rats. METHODS High-performance liquid chromatography method was established for determination of the concentrations of rapamycin and atorvastatin. Two models were set up to study the effects of drugs on the intestinal absorption. The model of everted gut sac in rats was adopted as in vitro dispersion test and the model of in situ single pass intestinal perfusion （SPIP） was chosen to collect perfusate sample. The sample concentrations of rapamycin and atorvastatin were measured by HPLC. RESULTS The intestinal permeation amounts of rapamycin and atorvastatin concentrations were increased significantly after co-administration. CONCLUSION The above findings reveal that the absorption of rapamycin and atorvastatin in small intestinal tract was enhanced after co-administration.

关 键 词：雷帕霉素 阿托伐他汀 HPLC 小肠吸收 

分 类 号：R966[医药卫生—药理学]