知母皂苷A Ⅲ对人脐静脉内皮细胞增殖和凋亡的影响  

Effects of Timosaponin A Ⅲ on the Proliferation and Apoptosis of Human Umbilical Vein Endothelial Cells

作　　者：潘会君[1,2] 莫小辉 冯彦军[4] 陈中建 陈松 秦路平[2] 

机构地区：[1]上海市皮肤病医院药剂科,上海200443 [2]第二军医大学药学院生药教研室,上海200433 [3]上海市皮肤病医院中心实验室,上海200443 [4]苏州市第五人民医院,江苏苏州215000

出　　处：《中国药房》2014年第43期4049-4051,共3页China Pharmacy

基　　金：上海市皮肤病医院青年人才培养计划(No.2013)

摘　　要：目的:研究知母皂苷AⅢ对人脐静脉内皮细胞(HUVECs)增殖和凋亡的影响。方法:体外培养HUVECs,分别以浓度为0、1、2、4、8μmol/L的知母皂苷AⅢ作用24、48、72 h,通过MTT比色法检测细胞生长程度;倒置显微镜观察细胞形态。流式细胞仪检测细胞凋亡情况。结果:8μmol/L知母皂苷AⅢ作用24、48、72 h可明显抑制HUVECs增殖,IC50分别为7.2、4.0、2.0μmol/L;随着知母皂苷AⅢ作用时间的延长和浓度的增加,HUVECs数目逐渐减少,细胞开始出现空泡、固缩现象;8μmol/L知母皂苷AⅢ作用于HUVECs,细胞凋亡率为(4.03±0.41)%。结论:知母皂苷AⅢ可明显抑制HUVECs的生长,并诱导部分细胞发生凋亡。OBJECTIVE： To study the effect of timosaponin AⅢ on the proliferation and apoptosis of human umbilical vein en- dothelial cell （HUVEC）. METHODS：HUVEC were cultured in vitro and treated with 0,1, 2, 4 and 8 μmol/L timosaponin AIR for 24, 48 and 72h. MTT assay was adopted to detect the cell growth. The morphological changes of HUVECs were observed by invert- ed microscope. The apoptosis of HUVECs were detected by Annexin V-FITC/PI assay. RESULTS ：Timosaponin AIR （8 μmol/L） significantly inhibited the proliferation of HUVECs after treated for 24, 48 and 72 h. IC50 were 7.2, 4.0 and 2.0 μmol/L. The morphological observation of inverted microscope showed than the number of HUVECs was decreased as the duration of timosaponin AⅢ treatment and its dose were increased; cell vacuoles and pycnosis were found. The flow cytometry showed that apoptosis rate of HUVEC was （4.03 ± 0.41）% after treated with 8 μmol/L timosaponin AⅢ （P〈0.01）. CONCLUSIONS：Timosaponin AⅢ can sig- nificantly inhibit the proliferation of HUVECs and induced the apotosis of HUVECs.

关 键 词：知母皂苷A Ⅲ 人脐静脉内皮细胞 生长抑制 凋亡 

分 类 号：R285.5[医药卫生—中药学]