HPLC法同时测定舒肝解郁胶囊中金丝桃苷和芦丁的含量  

Simultaneous Determination of Rutin and Hyperoside in Shugan Jieyu Capsule by HPLC

作　　者：乔怀耀[1] 罗荣[1] 吴娟[1] 朱文漓[1] 柯尊洪[1] 郝晓锋[1] 柯潇[1] 邬智刚 

机构地区：[1]成都康弘药业集团股份有限公司,成都610036

出　　处：《中国药房》2014年第43期4079-4081,共3页China Pharmacy

基　　金：四川省重点技术创新项目(No.2012CD00082);四川省科技成果转化项目(No.2012SC002)

摘　　要：目的：建立同时测定舒肝解郁胶囊中金丝桃苷、芦丁含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent Eclipse Plus C18（250 mm×4.6 mm,5μm）,流动相为乙腈-甲醇（90∶10,V/V）-50 mmol/L磷酸盐缓冲液（梯度洗脱）,流速为0.8 ml/min,检测波长为360 nm,柱温为室温。结果：金丝桃苷和芦丁的进样量分别在1.272~-127.240、1.393-139.350μg范围内与峰面积积分值呈良好线性关系（r=0.9999）;二者精密度、稳定性、重复性的RSD〈2%;平均加样回收率分别为96.9%、98.6%（n均为9）。结论：该方法快速、准确,重复性好,可用于舒肝解郁胶囊的质量控制。OBJECTIVE： To develop a method for simultaneous determination of rutin and hyperoside in Shugan jieyu capsule. METHODS：HPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18（250 mm×4.6 mm, 5μm） column with mobile phase consisted of acetonitrile-methanol （90： 10, V/V）-50 mmol/L phosphate buffer （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelength was set at 360 nm, and the column temperature was room temperature. RESULTS： The linear ranges were 1.272-127.240μg for hyperoside and 1.393-139.350μg for rutin, respectively （r=0.999 9）. RSDs of precision, stability and reproducibility tests were all lower tharn 2%. The average recoveries were 96.9% and 98.6% （n=9）, respectively. CONCLUSIONS ： The method is rapid, accurate and repeatable, and can be used for quality control in Shugan jieyu capsule.

关 键 词：舒肝解郁胶囊 芦丁 金丝桃苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R283.65