分枝杆菌噬菌体Chy3对人气道上皮细胞16HBE生长及功能的影响  

Effects of mycobacteriophage Chy3 on growth and function of human airway epithelial cell 16HBE

作　　者：熊欣[1] 甘易玲[1] 徐莉[1] 江莉莎[1] 张莉[1] 郭述良[1] 

机构地区：[1]重庆医科大学附属第一医院呼吸内科,重庆400016

出　　处：《重庆医科大学学报》2014年第9期1202-1206,共5页Journal of Chongqing Medical University

基　　金：国家"十二.五"科技重大专项基金资助项目(编号:2012ZX10003009);国家临床重点专科专项经费资助项目(卫办医政函【2012】949号)

摘　　要：目的:探讨不同滴度的分枝杆菌噬菌体Chy3对人气道上皮细胞16HBE生长及功能的影响。方法:不同滴度噬菌体Chy3作用于人气道上皮细胞后,采用倒置显微镜观察细胞生长形态变化,CCK-8法检测细胞存活率,流式细胞仪检测细胞凋亡率,ELISA法检测细胞上清液中细胞因子分泌水平,RT-PCR和Western blot分别检测上皮细胞黏蛋白MUC5AC mRNA和蛋白表达水平。结果:不同滴度噬菌体Chy3作用于人气道上皮细胞一段时候后,细胞的生长形态、存活率、凋亡率、细胞因子分泌水平以及黏蛋白MUC5AC表达与正常组比较差异均无统计学意义(P>0.05)。结论:分枝杆菌噬菌体Chy3无论高低滴度对体外培养人气道上皮细胞生长和功能均无明显影响,有较好的安全性。Objective：To explore the effects of different titers of mycobacteriophage Chy3 on growth and function of human airway epithelial cell 16 HBE. Methods：Inverted microscope was used to observe cellular morphology after applying different titers of mycobacteriophage Chy3. Cell viability and apoptosis rates were analyzed by CCK-8 assay and flow cytometry. Additionally,the secretion levels of IL-6/IL-8 in cell culture supernatant were detected by ELISA. RT-PCR and Western blot were performed to detect MUC5 AC mRNA and protein expression. Results：There was no difference in cellular morphology,cell viability,apoptosis rate,secretion levels of IL-6/IL-8,MUC5 AC mRNA and protein expression between cells treated with different titers of Chy3 and normal controls（P 0.05）.Conclusion：Neither high nor low titer of mycobacteriophage Chy3 exerts effects on growth and function of human airway epithelial cell 16 HBE in vitro,therefore mycobacteriophage has better security.

关 键 词：分枝杆菌噬菌体 人气道上皮细胞 安全性 白细胞介素-6/白细胞介素-8 MUC5AC 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]