HAP-GEL支架复合成骨细胞修复兔颅骨缺损的实验研究  

Experiment study on the repair of rabbit skull defect with HAP-GEL scaffolds combined with cultured osteoblasts

作　　者：耿海霞[1] 郭秀娟[1] 钱君荣[1] 封伟[1] 

机构地区：[1]济宁医学院附属医院口腔科,山东济宁272000

出　　处：《上海口腔医学》2014年第5期539-542,共4页Shanghai Journal of Stomatology

基　　金：山东省自然科学基金(ZR2010HL046);山东省卫生厅科技发展计划项目(2011HZ018)~~

摘　　要：目的:对羟基磷灰石/凝胶纳米支架(HAP-GEL)复合成骨细胞修复兔颅骨缺损进行组织学评价。方法:将HAP-GEL新型仿生复合物与成骨细胞联合培养,制备兔颅顶骨骨缺损模型,植入HAP-GEL复合成骨细胞,空白骨缺损为阴性对照,自体颅骨为阳性对照。于术后4、8、12周,通过大体标本观察、X线或CT以及组织学观察分析骨缺损修复情况。结果:术后4、8、12周,影像学评估空白缺损均为明显透射影,HAP-GEL复合成骨细胞与自体颅骨修复均为高密度影,骨缺损中央密度接近周围正常骨组织,缺损边缘略显模糊。组织学观察,随着时间延长,材料降解且有新生骨小梁产生。结论:HAP-GEL支架复合成骨细胞在兔颅骨缺损中诱导新骨组织生成,可取得较好的修复效果。PURPOSE： To investigate the ability of composite graft of osteobalsts and HAP-GEL scaffolds to repair cranial bone defect. METHODS： HAP-GEL and osteoblasts were co-cultured.The rabbit cranial parietal bone defect models were established. HAP-GEL scaffolds and osteoblasts were implanted into the cranial bone defects with blank defect as negative control, autologous skull as positive control. After 4,8,12 weeks, the result was evaluated by gross inspection, three-dimensional computed tomographic scanning and histological examination, respectively. RESULTS： After 4, 8, 12 weeks, blank defects displayed significantly radiolucent area, HAP-GEL with osteoblasts and autologous skull restoration showed high density area, the edges were slightly blur. With time going by, part of the scaffolds were absorbed, and the new bone trabecula was observed in histological examination. In the control groups, only fibrous tissues were observed in the defect region, there was no new bone formation. CONCLUSIONS： The engineering bone constructed by osteoblasts and HAP-GEL scaffold can be applied to repair bone defect. Supported by Natural Science Foundation of Shandong Province （ZR2010HL046） and Project of Science and Technology Plan of Shandong Province （2011HZ018）.

关 键 词：羟基磷灰石/凝胶纳米复合物 成骨细胞 骨缺损 支架材料 兔 

分 类 号：R318.08[医药卫生—生物医学工程]