不同动脉压力感受器对钌红反应的敏感性差异  

Distinct sensitivity of different arterial baroreceptors on ruthenium red

作　　者：赵海燕[1] 黄海霞[2] 刘萍[2] 张思韬[2] 钮伟真[2] 王伟[2] 

机构地区：[1]首都医科大学燕京医学院机能教研室,北京100069 [2]首都医科大学基础医学院生理学与病理生理学系,北京100069

出　　处：《首都医科大学学报》2014年第5期640-645,共6页Journal of Capital Medical University

基　　金：国家自然科学基金(31371162);北京市优秀人才培养资助项目(D类2013D005018000001);首都医科大学燕京医学院科研启动基金(2014QD01)~~

摘　　要：目的研究钌红对压力感受器不同纤维的作用是否存在差异以及这些差异与感受器纤维类型的关系。方法制备家兔离体颈动脉窦-窦神经标本,采用颈动脉窦窦内外双重灌流,钳制窦内压同时记录窦神经细束(含少量纤维)放电,然后应用Spike2软件分析,获得单纤维放电的信息。结果在20μmol/L钌红作用下,根据对钌红的反应,可将窦神经压力感受纤维分为钌红敏感纤维和钌红不敏感纤维2类。进一步分析发现这2类纤维的电生理特点分别与C类和A类压力感受器相对应。结论窦神经中存在对钌红反应不同的2类纤维,可能分属A类纤维和C类纤维,提示这2类压力感受器的机-电换能机制可能存在某些差异。Objective To investigate if ruthenium red（ RR） possesses differentiating effects on baroreceptors and to understand the relationship between these different effects and types of the baroreceptors. Methods Carotid sinus-nerve preparation of the rabbit was perfused intrasinusally and extrasinusally in vitro. Baroreceptor discharges were recorded from nerve filaments containing 2-5 fibers while the intrasinusal pressure was clamped with pulsatile or ramp waves. Single fiber discharges were obtained by sorting the few-fiber discharge using software Spike 2. Results According to the response to RR（ 20 μmol /L）,baroreceptor fibers can be distinguished into two types： RR sensitive and insensitive fibers. To further analyze electrophysiological characteristics of the two types,we found that the RR sensitive and insensitive fibers correspond with C-and A-fibers,respectively. Conclusion Carotid sinus fibers can be classified into two types according to the response to RR（ 20 μmol /L）. The two types of baroreceptors might belong to A-and C-fibers,respectively. The results suggested that the mechanisms for mechano-electrical transduction in the two types of baroreceptor fibers might not be the same.

关 键 词：动脉压力感受器 钌红 钌红敏感纤维 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学]