接骨草的显微结构及其绿原酸组织化学定位研究  

Anatomical Structure and Histochemical Localization of Chlorogenic Acid of Sambucus chinensis Lindl.

作　　者：李爱民[1,2,3] 周红灿[1,2,3] 张晗[1] 平晶耀 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《西北植物学报》2014年第10期1999-2003,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：湖南省教育厅科研项目(10C1062);湖南省重点学科建设项目(2011-42);湖南省科技计划重点项目(2013FJ4324)

摘　　要：运用石蜡切片法和荧光显微镜观察法研究了3个不同接骨草(Sambucus chinensis Lindl.)居群营养器官的显微结构及其绿原酸的分布规律。结果表明:(1)接骨草地上茎厚角组织明显,髓部由大小不等的两类薄壁细胞组成,且有单宁细胞分布;地下根状茎厚角组织细胞小,髓部薄壁细胞大小差异不明显,皮层及髓中有油细胞分布。(2)叶片为异面叶,栅栏组织细胞为短柱状,油细胞不明显。(3)绿原酸分布在根状茎皮层部分细胞、茎厚角组织部分细胞及叶片的海绵组织中,以海绵组织中含量最高。研究认为,髓部薄壁细胞大小的差异可作为接骨草的一个鉴别特征;荧光显微镜观察法可迅速准确显示绿原酸的分布;在所研究的3个接骨草居群中,怀化居群的绿原酸含量最高,若以绿原酸为有效成分来采收接骨草,可以只采收叶。Anatomical structure of Sambucus chinensis Lindl. and histochemical localization of chlorogenic acid were studied by paraffin method and fluorescence observation in three populations. The results showed that： （1）There were obviously cottenchyma and tannin cells in stems; pith was composed of two types of parenchyma cells with varying sizes. Collenchyma cells were smaller in rhizome than that in stem;pith pa-renchyma cell size difference was not obvious;the oil cells distributed in the cortex and pith. （2）The leaf belonged to typical dorsi-ventral leaf the palisade tissue cells were short columnar; oil cell was not obvious. （3）Chlorogenic acid located in the cortex cells of rhizomes,some collenchyma cells of stern and spongy tissue of leaf, with the highest levels in spongy tissue. Differences of pith parenchyma cell size can be used as a distinction character of S. chinensis. Fluorescence microscope observation can be quickly and accurately display the distribution of chlorogenie acid. The highest content of chlorogenic acid was Huaihua population. If the chlorogenic acid as the effective component of S. chinensis we suggested that only the leave.

关 键 词：接骨草 显微结构 绿原酸 组织化学定位 

分 类 号：Q944.5[生物学—植物学]