丹参酮ⅡA磺酸钠抑制膀胱出口部分梗阻大鼠a-SMA的表达及其机制研究  

Study on the inhibitory effect of sodium tanshinoneⅡA sulfonate on a-SMA expression in PBOO rats and its mechanism

作　　者：姜晓晓[1,2,3] 王博[1] 彭云鹏[2] 朱海涛[2] 陈仁富[2] 孙晓青[1,2,3] 

机构地区：[1]徐州医学院,江苏徐州221000 [2]徐州医学院附属医院泌尿外科 [3]徐州医学院泌尿外科研究室

出　　处：《临床泌尿外科杂志》2014年第11期1022-1026,共5页Journal of Clinical Urology

基　　金：徐州市科技发展基金(编号XF11C066)

摘　　要：目的:观察丹参酮ⅡA磺酸钠(STS)对膀胱出口部分梗阻(PBOO)大鼠膀胱中a-SMA表达的影响,探究其可能的作用机制。方法:通过膀胱颈部分结扎法建立大鼠PBOO模型,24只SD大鼠随机分为PBOO组、STS治疗组(STS组)以及假手术组(Sham组),每组8只。STS组每天给予STS腹腔注射(10 mg/kg),持续4周。PBOO组及Sham组给予等量生理盐水腹腔注射。应用透射电镜及Masson染色检测膀胱组织中胶原纤维的表达,免疫组化和RT-PCR检测膀胱中a-SMA的蛋白及mRNA的表达,应用Western blot检测膀胱组织中ERK1/2、P-ERK1/2蛋白的表达。结果:PBOO组胶原纤维表达量高于Sham组(P<0.05),STS组胶原纤维表达量低于PBOO组(P<0.05)。PBOO组a-SMA蛋白及mRNA表达高于Sham组(P<0.05),STS组低于PBOO组(P<0.05)。三组ERK1/2蛋白表达差异无统计学意义(P>0.05),P-ERK1/2蛋白的表达,PBOO组高于Sham组,STS组低于PBOO组(P<0.05)。结论:STS能够抑制PBOO大鼠a-SMA的表达,其机制可能是通过抑制MAPK通路中ERK1/2蛋白的活化实现的。Objective：To observe the inhibitory effect of sodium tanshinoneⅡ A sulfonate（STS）on a-smooth muscle actin（a-SMA）in partial bladder outlet obstruction（PBOO）rats’ bladder and to explore its possible mechanism.Method：Establishment of PBOO rats models were made by partial ligation of bladder neck.Twenty-four SD rats were randomly divided into PBOO group,STS group and Sham group on average.STS group was given intraperitoneal injection of STS（10mg/kg）for four weeks,and PBOO group and Sham group were given normal saline by intra-peritoneal injection.Collagen fiber of bladder tissue was detected using transmission electron microscope and Masson staining.Then,the expression of a-SMA protein and mRNA were detected by immunohistochemistry and RT-PCR,and expression of ERK1/2,P-ERK1/2protein was explored by Western blot.Result：Collagen expression of PBOO group was higher than that of Sham group,and STS group was lower than PBOO group（P 〈0.05）.The expression of a-SMA protein and mRNA of PBOO group were higher than those of Sham group（P 〈0.05）,while STS group was lower than PBOO group（P 〈0.05）.However,the expression of ERK1/2protein among three groups showed no significant differences（P 〉0.05）.The expression of P-ERK1/2protein of PBOO group was higher than that of Sham group,and STS group was lower than PBOO group（P 〈0.05）.Conclusion：STS can inhibit the expression of a-SMA protein in PBOO rats,and its mechanism may be related to inhibition of ERK1/2protein activation in MAPK pathway.

关 键 词：丹参酮ⅡA磺酸钠 A-SMA 膀胱出口部分梗阻 

分 类 号：R694.2[医药卫生—泌尿科学]