似鲇高原鳅败血症病原菌的分离鉴定与病理学观察  

Isolation and identification of the pathogenic bacteria and the pathologic examination of septicemia in catfish-like loach (Triplophysa siluroides)

作　　者：周燕[1] 耿毅[1] 汪开毓[1] 阳磊[1] 刘丹[1] 徐敬钧 陈德芳[1] 黄小丽[1] 

机构地区：[1]四川农业大学动物医学学院,四川雅安625014

出　　处：《中国预防兽医学报》2014年第11期863-866,871,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：四川省科技支撑计划项目(2011N20071)

摘　　要：为确定一起似鲇高原鳅败血症疫情的病原,本实验通过无菌操作从病鱼体内分离到一株优势菌(GYQ),以腹腔注射与浸泡的方式进行人工感染试验,证明其为似鲇高原鳅败血症的病原菌。根据分离菌的形态、生理生化特性,结合16S r DNA和gyr B基因序列测定(KF952599和KJ001821)与系统发育分析,将其鉴定为鮰爱德华氏菌(E.ictaluri)。病鱼组织器官具有典型的败血症病理变化,以全身多组织器官充出血、细胞变性、坏死以及炎性细胞侵润为主要特征,尤其是肝、肾、脾、肠和脑较为严重。药敏结果显示该菌对氟苯尼考、强力霉素等6种药物表现为敏感;对庆大霉素等4种药物表现为不敏感。To identify the pathogenic agent caused the septicemia in catfish-like loach （Triplophysa siluroides）,a dominant bacteria was isolated from the diseased fish,and the bacteria was identified as Edwardsiella ictaluri （designated GYQ） according to the 16S rDNA and gyrase B subunit （gyrB） gene sequence alignments.In addition,histopathological examination showed hypememia and hemorrhage,degeneration,necrotic and the inflammatory response in many organs.Especially,the serious lesions occurred in the liver,kidney,spleen,gastrointestinal tract and brain.While,the antibiotic susceptibility test results indicated that the strain GYQ was sensitive to florfenicol,doxycycline and so on,but resistant to gentamicin,etc.

关 键 词：似鲇高原鳅 败血症 鮰爱德华氏菌 分离鉴定 病理损伤 

分 类 号：S852.61[农业科学—基础兽医学]