Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews  

作　　者：Gui LI Ren LAI Gang DUAN Long-Bao LYU Zhi-Ye ZHANG Huang LIU Xun XIANG 

机构地区：[1]Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, 650223, China [2]Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Chinese Academy of Sciences, Kunming Yunnan 650223, China [3]Yunnan Agricultural University, Kunming Yunnan 650201, China Chinese Academy of Sciences, Kunming Yunnan Yunnan Province, Kunming Institute of Zoology

出　　处：《Zoological Research》2014年第6期492-499,共8页动物学研究（英文）

基　　金：This study was supported by the National 863 Project of China （2012AA021801） and the Project of Frontier Study of Foundation, CAS （KSCX2-EW-R-11, KSCX2-EW-J-23）

摘　　要：Endosymbionts influence many aspects of their hosts’ health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews(Tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16 S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis(detection rate 42%), Pseudomonas aeruginosa(25%), Staphlococcus aureus(33%), S. Epidermidis(75%), Micrococcus luteus(25%), Kurthia gibsonii(17%); oral cavity: Neisseria mucosa(58%), Streptococcus pneumonia(17%); anus: Enterococcus faecalis(17%), Lactococus lactis(33%), Escherichia coli(92%), Salmonella typhosa(17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis(75%); oral cavity: N.mucosa(67%); anus: L. lactis(33%), E. coli(100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.Endosymbionts influence many aspects of their hosts＇ health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews （Tupaia belangeri chinensis） have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews： body surface： Bacillus subtilis （detection rate 42%）, Pseudomonas aeruginosa （25%）, Staphlococcus aureus （33%）, S. Epidermidis （75%）, Micrococcus luteus （25%）, Kurthia gibsonii （17%）; oral cavity： Neisseria mucosa （58%）, Streptococcus pneumonia （17%）; anus： Enterococcus faecalis （17%）, Lactococus lactis （33%）, Escherichia coli （92%）, Salmonella typhosa （17%）; whereas, four were indentified from the third generation captive tree shrews： body surface： S. epidermidis （75%）; oral cavity： N.mucosa （67%）; anus： L. lactis （33%）, E. coli （100%）. These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.

关 键 词：Tree shrew MICROBIAL Separation Identification 

分 类 号：Q95-33[生物学—动物学]