Genetic diversity and population structure of a Sichuan sika deer(Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation  

作　　者：Ya HE Zheng-Huan WANG Xiao-Ming WANG 

机构地区：[1]School of Life Sciences, East China Normal University [2]Shanghai Science & Technology Museum

出　　处：《Zoological Research》2014年第6期528-536,共9页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(30870308 and 31071944);the Shanghai Rising-Star Program(10QA1402200)

摘　　要：Cervus sichuanicus is a species of sika deer （Cervus nippon Group）. To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve （TNR） and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer （Cervus hortulorum） released in TNR.Cervus sichuanicus is a species of sika deer(Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve(TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After Gene Scan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer(Cervus hortulorum) released in TNR.

关 键 词：Sichuan sika deer MICROSATELLITE Genetic diversity Population structure 

分 类 号：Q953[生物学—动物学]