阴行草水煎液对肝纤维化大鼠的肝保护作用  

Liver protective effects of Siphonostegia chinensis decoction in rats with liver fibrosis

作　　者：潘金[1] 毕洪钟 王伟[1] 琚坚[1] 

机构地区：[1]昆明医科大学第二附属医院特需病房,650101

出　　处：《国际中医中药杂志》2014年第11期1002-1005,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的：探讨阴行草水煎液对四氯化碳（CCl4）诱导的肝纤维化大鼠的肝脏保护作用。方法120只雄性SD大鼠随机分为空白对照组、肝纤维化模型组、阴行草组、丹参组、虎杖组及易善复组，每组20只。除空白对照组外，其余5组以CCl4皮下注射诱导肝纤维化；除空白对照组、肝纤维化模型组外，其余4组大鼠分别给予阴行草、丹参、虎杖及易善复10 ml/kg灌胃，1次/d。10周后，采用酶联免疫吸附法检测血清肿瘤坏死因子-α（TNF-α）水平；光镜下观察肝脏病理组织学改变，并进行肝纤维化分期。结果与肝纤维化模型组比较，阴行草组可降低肝纤维化程度（P＜0.01），且阴行草组、虎杖组、丹参组和易善复组之间肝纤维化组织学分期无显著差异（P＞0.05）。与肝纤维化模型组 TNF-α浓度（368.06±24.90）U/L比较，阴行草组（336.61±20.20）U/L明显降低（P＜0.01）；阴行草组与丹参组（337.81±21.04）U/L、虎杖组（338.95±24.43）U/L、易善复组（337.11±23.64）U/L比较差异均无统计学意义（P＞0.05）。结论阴行草水煎液对CCl4诱导的肝纤维化大鼠肝脏有保护作用。Objective To investigate the liver protective effects of Siphonostegia chinensis decoction in rats with liver fibrosis induced by carbon tetrachloride（CCl4）.Methods A total of 120 male SD rats were randomly divided into six groups： a blank control group, a model group, a Siphonostegia chinensis decoction group, a Salvia miltiorrhiza decoction group, a Rhizoma polygoni cuspidati decoction group and a polyene phosphatidylcholine capsules group, with 20 rats in each group. The model of liver fibrosis in rats was induced by subcutaneous injection with CCl4, except the blank group. Except rats in the blank control group and the model group, the rats in the other four groups were treated with Siphonostegia chinensis decoction, Salvia miltiorrhiza decoction, Rhizoma polygoni cuspidati decoction and polyene phosphatidylcholine capsules（10 ml/kg）. The serum level of tumor necrosis factor-α（TNF-α）was determined with enzyme-linked immunosorbent assay, the fibrosis stage assessment were determined by histopathological examination.Results Compared with the model group, Siphonostegia chinensis decoction group significantly reduced the stage of liver fibrosis（P〈0.01）and there were no obvious difference in liver fibrosis stage among the Salvia miltiorrhiza decoction group, Rhizoma polygoni cuspidati decoction group and polyene phosphatidylcholine capsules group, and the Siphonostegia chinensis decoction group（allP〉0.05）.Compared with liver fibrosis model group（368.06±24.90）U/L, the serum level of TNF-α in the Siphonostegia chinensis decoction group（336.61± 20.20）U/L significantly reduced（P〈0.01）, and there were no obvious difference among the Siphonostegia chinensis decoction group, the Salvia miltiorrhiza decoction group（337.81±21.04）U/L, the Rhizoma polygoni cuspidati decoction group（338.95±24.43）U/L and the polyene phosphatidylcholine capsules group（337.11± 23.64） U/L（allP〉0.05）.Conclusion Siphonostegia chinensis decoction has certain liver protective effect in

关 键 词：阴行草 肝纤维化 药物性肝损伤 四氯化碳 肿瘤坏死因子-α 防护药(中药) Tumor NECROSIS Factor-α Protective Agents(TCD) 

分 类 号：R285.5[医药卫生—中药学]