聚吡咯/PLGA可导电复合纳米纤维支架对肝细胞生物相容性的研究  

Biocompatibility study of polypyrrole/PLGA conductive nanofiber scaffold on hepatocytes

作　　者：褚薛慧[1] 许茜[2] 冯章启[3] 肖江强[1] 李强[1] 孙喜太[1] 曹阳[2] 丁义涛[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肝胆外科,210008 [2]东南大学生物电子学国家重点实验室,南京210096 [3]中山大学工学院,广州510006

出　　处：《国际生物医学工程杂志》2014年第5期271-274,298,共5页International Journal of Biomedical Engineering

基　　金：国家自然青年科学基金资助项目（8110116）

摘　　要：目的 评估聚吡咯/PLGA可导电复合纳米纤维支架对肝细胞的生物相容性.方法 采用静电纺丝及氧化结合法制备聚吡咯/PLGA可导电复合纳米纤维支架,并对其进行电镜表征及电化学检测.采用2步原位胶原酶法分离培养大鼠原代肝细胞.实验组将大鼠肝细胞培养于聚吡咯/PLGA可导电复合纳米纤维支架表面,而对照组设为PLGA培养组及空白对照组;采用MTT法检测各组细胞活性,BCA试剂盒评估各组细胞数量,ELISA法检测白蛋白分泌及尿素合成,同时还测定了各组肿瘤坏死因子-α（TNF-α）及乳酸脱氢酶（LDH）的浓度.结果 MTT实验及BCA检测提示聚吡咯/PLGA组与PLGA组间细胞数量及活性差异无统计学意义,但相比空白对照组其差异均具有统计学意义（P＜0.05）.白蛋白分泌及尿素合成方面也有类似表现,聚吡咯/PLGA组与PLGA组间差异无统计学意义,但较空白对照组要好.各组TNF-α及LDH的浓度差异无统计学意义（P＞0.05）.结论 聚吡咯/PLGA可导电复合纳米纤维支架具有良好的生物相容性,可以作为肝脏组织工程中肝细胞黏附介质的理想材料.Objective To evaluate the biocompatibility of polypyrrole/PLGA conductive nanofiber scaffold on hepatocytes.Methods The polypyrrole/PLGA conductive nanofiber scaffold was designed via electrospinning and oxidative polymerization,and characterized by scanning electron microscope （SEM） and cyclic voltammetry.Rat hepatocytes were harvested by a two-step in situ collagenase perfusion procedure.Rats were divided as polypyrrole/PLGA group,PLGA group and the blank control group.The cytoactivity was detected by MTT assay,cell number was assessed by BCA kit,the albumin secretion and urea synthesis were detected by enzyme-linked immunosorbent assay （ELISA）,and the concentration of lactate dehydrogenase （LDH） and tumor necrosis factor-α （TNF-α） was also measured.Results There was no significant difference in the cell number and cytoactivity between polypyrrole/PLGA group and PLGA group （P＞0.05）,which were both better than the blank control group （P＜0.05）.The urea synthesis and albumin secretion of hepatocytes on polypyrrole/PLGA conductive scaffold had a similar phenomenon.There was no significant difference between the concentration of LDH and TNF-α among the three groups （P＞0.05）.Conclusions The polypyrrole/ PLGA conductive nanofiber scaffold shows good biocompatibility,which suggests its potential application as a suitable substratum for hepatocytes culturing in liver tissue engineering.

关 键 词：聚吡咯 肝细胞 纳米支架 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] Q813[医药卫生—基础医学]