650 nm低能激光对高胆固醇家兔模型血液红细胞的影响研究  

Effect of erythrocytes in hypercholesterolemia rabbits irradiation by low level 650 nm laser

作　　者：王宏[1] 吴小光 王红星 陈洪丽[1] 李迎新[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192 [2]正安(北京)医疗设备有限公司,北京100007

出　　处：《国际生物医学工程杂志》2014年第5期295-298,共4页International Journal of Biomedical Engineering

基　　金：中央高校基本科研业务费专项资金资助项目;北京协和医学院协和青年基金项目（33320140056）

摘　　要：目的 研究650 nm低能激光对高胆固醇家兔耳廓根部进行照射对其血液红细胞结构和功能的影响.方法 选择16只雄性家兔,给予家兔高脂饲料,制备高胆固醇症家兔模型,将造模成功家兔随机分为治疗组和模型组,每组6只;另选择6只血脂正常动物作为对照组.治疗组采用650 nm半导体激光进行耳廓根部照射,激光能量100 mW,照射频率：2次/d,30 min/次,6d/周,共治疗16周.正常对照组、模型组不做处理.每4周采集耳缘静脉血液进行血液常规和生化分析,治疗结束后做血液滴片镜下观察红细胞聚集状态.结果 与对照组比较,治疗组红细胞总数降低,模型组红细胞数量偏高,3组差异有统计学意义（P＜0.05）.血红蛋白含量各组间差异无统计学意义（P＞0.05）.治疗组红细胞压积较对照组略低,模型组压积较对照组偏高;模型组红细胞粘连程度较高,而治疗组红细胞粘连聚集程度有所改善,但血液中总胆红素含量偏高.结论 大剂量低能激光血液照射对高血脂家兔的红细胞数量、细胞压积及聚集和黏附状态有影响,且可能对红细胞有一定的破坏作用,引起总胆红素的升高.Objective To study the structure and function changes of erythrocyte in hypercholesterolemia rabbits that treated with irradiation in auricle root by low level 650 nm laser.Methods Sixteen male rabbits were selected to establish the model animal of hypercholesterolemia,successful model rabbits were divided into 2 groups （model and treatment groups）,6 rabbits per group,and 6 normal rabbits were selected as control group.650 nm laser of 100 mW were given in auricle root in rabbits of treatment group for 16 weeks （6 d one week,twice a day,30 min one time）.Blood from ear vein was collected every 4 weeks to perform routine and biochemical blood examination,and one-drop blood test under microscope was used for blood accumulation observation.Results Total number of red blood cells of treatment group decreased,while model group showed an increasing trend than normal group （P＜0.05）.Hemoglobin in three groups were lower than other two groups （P＞0.05）.Hematocrit （HCT） in treatment group were lower than that in normal group,while HCT in model group were higher than that in other two groups and showed a higher aggregation degree also.Less than model group,treatment group showed less aggregation degree but with higher total bilirubin.Conclusions Low level laser of 650 nm showed an effect on total number of erythrocytes,HCT and red blood cells aggregation,high dose of laser might be injury for erythrocytes and lead to total bilirubin increasement.

关 键 词：低能激光 红细胞 血液照射 高胆固醇症 

分 类 号：R454.2[医药卫生—治疗学] R589.2[医药卫生—临床医学]