miR-133调控靶基因HCN2在风湿性心脏病心房颤动中的作用机制  

The mechanism of miR-133regulates target genes HCN2 in rheumatic heart disease with atrial fibrillation

作　　者：付勇[1] 刘慧[2] 毛亮[3] 于风旭[1] 邓明彬[1] 李新[1] 廖斌[1] 

机构地区：[1]泸州医学院附属医院胸心外科,四川泸洲646000 [2]泸州医学院附属医院超声诊断科 [3]泸州医学院心肌电生理学研究室

出　　处：《临床心血管病杂志》2014年第10期890-893,共4页Journal of Clinical Cardiology

基　　金：泸州市科技局基金资助项目(No:2011-s-35);泸州医学院基金资助项目(No:2011-45);泸州医学院附属医院基金资助项目(No:2011-43)

摘　　要：目的:通过检测风湿性心脏病﹙风心病﹚心房颤动﹙房颤﹚患者右心耳组织miR-133及靶基因HCN2表达,探讨其和风心病房颤的可能关系。方法:10例风心病患者根据是否存在房颤,分为房颤组和窦性心律﹙窦律﹚组,术前均行心脏彩色多普勒超声与心电图检测,术中取右心耳组织,应用荧光定量PCR技术检测miR-133及靶基因HCN2的表达。结果:房颤组miR-133表达小于窦律组[﹙0.143 600±0.021 767)︰(0.215 800±0.023 510),P<0.05];而HCN2基因表达水平房颤组高于窦律组[﹙0.054 20±0.011 45)︰(0.030 40±0.003 29),P<0.05]。结论:风心病房颤患者miR-133表达降低,通过上调靶基因HCN2表达,可能参与风心病房颤的发生。Objective：Through examining the right atrial appendage organization miR-133 and target genes HCN2 expression in patients with rheumatic heart disease（RHD）and atrial fibrillation（AF）,to explore its possible relationship with AF.Method：Ten patients with RHD were divided into AF group（n=5）and sinus rhythm group（SR group,n=5）according to AF or not.The color Doppler ultrasound and ECG were examined preoperative,the right atrial appendage tissue were taken to detect miR-133 and HCN2mRNA expression using real time quantitative poly-merase chain reaction（RT-qPCR）.Result：There were significant differences in miR-133 and HCN2expression levels between the SR group and AF group.The miR-133 expression levels of the former was higher than the latter[（0.215 800±0.023 510）vs（0.143 600±0.021 767）,P〈0.05],but the HCN2 mRNA expression levels of the former was lower than the latter[（0.030 40±0.003 29）vs（0.054 20±0.011 45）,P〈0.05].Conclusion：Downregulation of miR-133 upregulate HCN2expression levels in RHD with AF,which may be a correlation with the occurrence of RHD AF.

关 键 词：风湿性心脏病 窦性心律 心房颤动 miR-133 超极化激活环核苷酸门控阳离子通道-2 

分 类 号：R541.2[医药卫生—心血管疾病]