海洋真菌Aspergillus sp.SCSGAF 0076与细菌Bacillus sp.MNMCCE001共培养次生代谢产物  

Secondary metabolites of the co-culture of Aspergillus sp.SCSGAF 0076 and Bacillus sp. MNMCCE 001

作　　者：董杰杰 张晓勇[1] 鲍洁[1,2] 徐新亚[1] 农旭华[1] 漆淑华[1] 

机构地区：[1]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,广东广州510301 [2]中国科学院大学,北京100049

出　　处：《微生物学报》2014年第11期1289-1295,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目(41376160);广东省海洋经济创新发展区域示范专项(GD2012-D01-002)~~

摘　　要：【目的】阐明海洋真菌Aspergillus sp.SCSGAF 0076与细菌Bacillus sp.MNMCCE 001在平板上共培养时产生的主要抗菌物质和红色素的化学结构。【方法】在固体淀粉培养基上分别进行Aspergillus sp.SCSGAF 0076纯培养、以及SCSGAF 0076和Bacillus sp.MNMCCE 001共培养,培养3 d后分别对纯培养和共培养的培养基进行萃取浓缩得到浸膏,运用高效液相色谱分析比较纯培养和共培养所得浸膏的化学分成的差异,并采用抗菌活性追踪法,结合硅胶柱层析、凝胶柱层析和高效液相色谱等分离方法从共培养浸膏中分离纯化抗菌物质和红色素,运用波谱解析法鉴定化合物的结构。【结果】通过高效液相色谱分析发现,共培养与纯培养的主要次生代谢产物没明显差异,但抗菌物质和红色素的含量差别明显。从这两株菌共培养的发酵产物中分离鉴定了4个化合物,包括抗菌物质青霉酸(1)、青霉酸类似物5(6)-dihydropenicillic acid(2)和9-chloro-8-hydroxy-8,9-deoxyasperlactone(3)、以及红色素viopurpurin(4)。【结论】初步阐明了Aspergillus sp.SCSGAF 0076与Bacillus sp.MNMCCE 001共培养时由Aspergillus sp.SCSGAF 0076产生的主要抗菌活性化合物是青霉酸,伴随明显增多的主要红色素是viopurpurin,二者产率均比Aspergillus sp.SCSGAF 0076纯培养时高。[ Objective ] To elucidate the chemical structures of the main antibacterial compound and red pigment produced by the co-culture of marine-derived fungus Aspergillus sp. SCSGAF 0076 and bacterium Bacillus sp. MNMCCE 001. [ Methods] The monocuhure of strain SCSGAF 0076 and co-culture of strains SCSGAF 0076 and MNMCCE 001 were done on amylum solid medium for three days, then the crude extracts of the cultures were obtained, and subsequently, the chemical profiles of the extracts of monoculture and co-culture were analyzed by HPLC. Using antibacterial bioassay- guided fractionation, we isolated the crude extract of the co-culture by silica gel column chromatogram, Sephadex LH-20, and semi-preparative HPLC to obtain the main antibacterial compound and red pigment. The compounds＇ structures were determined by spectroscopic analysis. [ Results ] We found that the main secondary metabolites produced by the monoculture of strain SCSGAF 0076 and co-culture of strains SCSGAF 0076 and MNMCCE 001 were almost the same, however, the contents of the main antibacterial compound and red pigment were obviously different. Totally, four compounds including the antibacterial compound penicillic acid, 5 （6）-dihydropenicillic acid, 9-chloro-8-hydroxy-8,9- deoxyasperlactone and red pigment viopurpurin were isolated from the crude extract of the co-culture medium. [Conclusion] The main antibacterial compound of the co-culture of strains SCSGAF 0076 and MNMCCE 001was penicillic acid, the main red pigment was viopurpurin, and the yields of the two compounds were proved by the co- culture.

关 键 词：真菌Aspergillus sp. 细菌Bacillus sp. 共培养 抗菌 青霉酸 红色素 

分 类 号：Q936[生物学—微生物学]