槲皮素对前列腺癌细胞热休克蛋白27表达的影响  

Effect of Quercetin on Heat Shock Protein 27 Expression in Prostate Cancer Cells

作　　者：于峰[1] 姜丽丽[1] 邸彦橙[1] 

机构地区：[1]牡丹江医学院红旗医院泌尿外科,黑龙江牡丹江157011

出　　处：《中国医学科学院学报》2014年第5期506-509,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的观察热休克蛋白27(HSP27)在不同恶性程度前列腺癌细胞株RWPE-1、LNCaP、PC-3和TSU-Pr1中的表达情况,分析槲皮素对PC-3细胞株HSP27表达的影响。方法体外培养人前列腺癌RWPE-1、LNCaP、PC-3和TSUPr1细胞株,分别分为以下4组:(1)空白对照组;(2)阴性对照组:给予二甲基亚砜;(3)槲皮素高剂量组:给予0.6 mg/ml槲皮素150μl;(4)槲皮素低剂量组:给予0.3 mg/ml槲皮素150μl。采用RT-PCR和免疫荧光染色法检测HSP27的表达情况。结果 RT-PCR和免疫荧光染色结果显示,HSP27的表达取决于细胞类型,按照RWPE-1、LNCaP、PC-3、TSU-Pr1的顺序依次增强。空白对照组、阴性对照组、槲皮素高剂量组和槲皮素低剂量组PC-3细胞的HSP27 mRNA表达水平分别为128%、110%、50%、60%,其中,槲皮素高剂量组和槲皮素低剂量组的表达均明显低于空白对照组(P均<0.05)。结论 HSP27的表达与前列腺癌恶性程度有关,槲皮素可抑制PC-3细胞中HSP27的表达。Objective To evaluate the mRNA expression of heat shock protein 27 （HSP27） in differ- ent prostate cancer cell lines including RWPE-1, LNCaP, PC-3, and TSU-Prl and to further analyze the effect of quercetin on PC-3 cell lines. Methods The in vitro cultured human prostate cancer cell lines RWPE-1, LN- CaP, PC-3, and TSU-Prl were randomly divided into four groups： control group; negative control group （trea- ted with dimethyl sulfoxide）, high-dose group （treated with 150 μl 0. 6 mg/ml quercetin）, and low-dose group （treated with 150 μl 0. 3 mg/ml quercetin） . The mRNA expression of HSP27 was detected by reverse transcrip- tion polymerase chain reaction （RT-PCR） and immunofluorescence staining. Results RT-PCR and immunoflu- orescence staining showed that HSP27 expression were highly dependent on the cell types and increased in an or- der of RWPE- 1, LNCaP, PC- 3, and TSU-Pr1 after quercetin treatment. The HSP27 mRNA expression levels were 128%, 110%, 50%, and 60%in control group, negative control group, high-dose group, and low-dose group. Obviously, it was significantly lower in the high-dose group and low-dose group than in the control group （ both P 〈 0. 05） . Conclusions HSP27 expression level is associated with the the degree of prostate cancer. Quercetin may inhibit HSP27 expression in PC-3 cell.

关 键 词：槲皮素 前列腺癌 热休克蛋白27 

分 类 号：R585.5[医药卫生—内分泌]