新型过氧化物酶peroxiredoxin-1对大鼠肺成纤维细胞JNK介导的Ⅰ和Ⅲ型前胶原合成的影响  

Effect of peroxiredoxin-1 on JNK-involved collagen type Ⅰ and Ⅲ expressions in TGF-β1-induced pulmonary fibroblasts

作　　者：孙影[1] 魏中秋[1] 胡亚萍[1] 郑素琴[1] 吴剑[2] 冯莉[1] 杨方[3] 

机构地区：[1]河北联合大学基础医学院病理学系,唐山063000 [2]唐山市妇幼保健院内科,唐山063000 [3]河北联合大学实验中心,唐山063000

出　　处：《解剖学杂志》2014年第5期590-593,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81072254）;河北省高等学校科学技术研究青年基金（Q2012090）

摘　　要：目的：探讨活性氧（ROS）在转化生长因子（TGF-β1）激活c-Jun N末端激酶（JNK）促进肺成纤维细胞合成Ⅰ和Ⅲ型前胶原过程中的作用,并观察新型过氧化物酶peroxiredoxin-1 （Prx-1）是否通过抑制活性氧来抑制TGF-β1促纤维化作用.方法：采用脂质体转染法转染真核质粒;免疫荧光检测质粒转染和8-OHdG（DNA氧化产物）的水平;免疫印迹检测Ⅰ/Ⅲ型前胶原、磷酸化JNK和Prx-1的表达.结果：与对照组比较,TGF-β1组的Ⅰ/Ⅲ型前胶原、8-OHdG及磷酸化p-JNK的表达水平均明显增加.与TGF-p1组比较,空载体组中的上述观察指标无明显变化,但Prx-1转染组的指标均明显下降.结论：TGF-β1能够诱导肺成纤维细胞生成活性氧,并由此促进JNK的激活和Ⅰ/Ⅲ型前胶原合成增加,而Prx-1则通过抑制活性氧来抑制TGF-β1的促纤维化作用.Objective： To explore the effect of reactive oxygen species （ROS） on TGF-β1-induced c-Jun N-terminal kinase （JNK） activation and collagen synthesis in cultured pulmonary fibroblasts, and to study whether peroxiredoxin-1 （Prx-1, a noval type of peroxidase） can inhibit these changes by decreasing ROS level. Methods： Prx-1 was transfected into pulmonary fibroblasts using the liposome infection. Immunofluorescence was used to evaluate plasmid transfection and 8-OHdG level; while the expressions of collagen type I and IN, phosphorylated JNK （p-JNK） and Prx-1 were measured by Western blotting. Results： The plasmids were successfully transfected into pulmonary fibroblasts, showing as green flurescence in cytoplasm and increased expression of Prx-1 protein compared with control （no plasmid） and negative control （plasmid without prx-1 gene）. TGF-β1 significantly stimulated the expressions of collagen type I and Ⅲ, p-JNK and 8-OHdG in pulmonary fibroblasts, which could be markedly inhibited by Prx-1 plasmid transfection but not in the vehicle group. Conclusion： TGF-β1 induces pulmonary fibroblasts to generate ROS, which contributes to JNK activation and increases collagen synthesis; however, Prx-1 overexpression inhibits these changes by decreasing ROS level.

关 键 词：peroxiredoxin-1 活性氧 C-JUN氨基末端激酶 转化生长因子-Β1 前胶原 

分 类 号：R135.2[医药卫生—劳动卫生]