ERK2和PI-3K在小柴胡汤抗大鼠肝纤维化中的表达  

Xiaochaihu Decoction treats liver fibrosis in rats by regulating expression of ERK2 and PI-3K

作　　者：李晋[1] 徐尚福[2] 罗果[1] 白国辉[1] 

机构地区：[1]遵义医学院医学与生物学研究中心,贵州遵义563000 [2]遵义医学院基础药理省部共建教育部重点实验室/药理学教研室,贵州遵义563000

出　　处：《中成药》2014年第11期2238-2242,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81360661);贵州省科学技术基金(黔科合J字LKZ[2010]43号)

摘　　要：目的观察小柴胡汤对肝纤维化大鼠肝组织细胞外信号调节激酶2(ERK2)、磷酸肌醇-3激酶(PI-3K)表达的影响,探讨小柴胡汤治疗肝纤维化的分子机制。方法皮下注射10%四氯化碳制备大鼠肝纤维化模型,第9周开始四氯化碳给药同时给予小柴胡汤灌胃治疗6周。通过血清生化检测反映肝脏功能;HE染色法观察肝组织病理学变化;免疫组织化学方法观察肝组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达,以反映肝星状细胞的活化情况;Real time RT-PCR观察ERK2、PI-3K mRNA的表达。结果与模型组相比,小柴胡汤中、高剂量组在血清学和组织学上均有明显改善,肝组织ERK2、PI-3K mRNA的表达明显减少,具有统计学意义。结论小柴胡汤抗四氯化碳诱导大鼠肝纤维化作用可能与干预Ras/ERK和PI-3K信号通路有关。AIM To observe the effect of Xiaochaihu Decoction on the expression of extracellular signal-regulated kinase 2 （ERK2） and phosphoinositide-3 kinase （PI-3 K） in rat with hepatic fibrosis and to explore their molecular mechanism.METHODS Liver fibrosis in rats was induced by subcutaneous injection of 10％ CCl4.Rats were then treated with oral Xiaochaihu Decoction for six weeks starting from the ninth week.Liver function was tested by serum biochemical reaction.Liver tissue was analyzed by HE staining.α-smooth muscle actin was observed by immunohistochemistry to reflect the activation of hepatic stellate cells.The expression of ERK2 and PI-3K was measured by real time RT-PCR.RESULTS Compared with the model group,the middle and high dose of Xiaochaihu Decoction groups significantly improved in serology and histology.They obviously reduced the expression of ERK2 and PI-3K with statistical significance.CONCLUSION Xiaochaihu Decoction in the treatment of CCl4-induced liver fibrosis may be related to the intervention in Ras/ERK and PI-3K signaling pathway.

关 键 词：肝纤维化 小柴胡汤 细胞外信号调节激酶2 (ERK2) 磷酸肌醇-3激酶(PI-3K) 

分 类 号：R966[医药卫生—药理学]