苁归益肾胶囊对糖尿病肾病大鼠肾脏Nephrin蛋白和Podocin蛋白表达的影响  

Effect of Conggui Yishen Capsules on the expression of Nephrin and Podocin in kideny of diabetic nephropathy rats

作　　者：段贤春[1] 方朝晖[1] 汪永忠[1] 高家荣[1] 吴健[1] 陈曦[1,2] 罗欢[1] 姚先梅[1] 李瑛[1] 朱红[1] 夏伦祝[1] 

机构地区：[1]安徽中医药大学第一附属医院,国家中医药管理局中药制剂三级实验室,安徽合肥230038 [2]安徽中医药大学药学院,安徽合肥230031

出　　处：《中成药》2014年第11期2248-2252,共5页Chinese Traditional Patent Medicine

基　　金：国家中医临床研究基地重点病种糖尿病专项基金项目(JDZX2012001);安徽省中医发展省级专项补助资金项目(卫中医药秘[2014]342号;2014ZYZJ02);国家中医药管理局重点学科-中医内分泌学学科开放基金项目(2011nfmxk011;2011nfmxk012);安徽中医学院临床科学研究基金项目(2010LC-003A);国家中医药重点学科临床中药学建设项目(国中医人教发【2012】32号)

摘　　要：目的探讨苁归益肾胶囊(由肉苁蓉、当归、山茱萸、桂枝、泽泻和丹皮组成)对高糖高脂饲料喂养加链脲佐菌素诱导大鼠糖尿病肾病肾脏足细胞裂孔隔膜蛋白相关分子(肾病蛋白Nephrin和肾小球足细胞裂隙膜蛋白Podocin)表达的影响,探讨其降低尿蛋白、保护肾脏的机制。方法随机抽取大鼠10只作为正常组,并将90只成模大鼠随机分为模型组、厄贝沙坦组、苁归治疗组(苁归益肾胶囊高、中、低剂量组)。治疗10周后,收集大鼠尿液测定24 h尿蛋白定量,肾脏HE染色观察病理损伤,检测各组大鼠肾功能,生物素双抗体夹心酶联免疫吸附法(ELISA)检测肾组织Nephrin和Podocin蛋白的表达。结果苁归益肾胶囊可改善糖尿病肾病大鼠肾组织病理损伤,降低糖尿病肾病大鼠24 h尿蛋白定量血肌酐和血尿素,并能增加肾组织中Nephrin和Podocin蛋白的表达。各治疗组肾组织病理改变较模型组均有不同程度的改善。结论苁归益肾胶囊对糖尿病肾病模型大鼠具有一定的治疗作用,其机制可能与上调Podocin和Nephrin表达有关。AIM To study the influence of Conggui Yishen Capsules （Cistanches Herba,Angelicae sinensis Radix,Corni Fructus,Cinnamomi Ramulus,Alismatis Rhizoma,Moutan Cortex） on the expression of Nephrin and Podocin in rat with diabetic nephropathy induced by high sugar,fatty food and streptozotocin injection.METHODS Ten rats were randomly chosen as normal control group,ninety rats with diabetic nephropathy were divided into five groups,including the model group,Irbesartan group,high,medium and low-dosage Conggui Yishen Capsules group.After ten weeks＇ treatment,24 h urinary protein in urine was collected and measured.HG staining of kidney tissue was used for pathological damage observation.The expression of Nephrin and Podocin were determined by ELISA.RESULTS After treatment,24 h urine protein,kidney weight index,serum creatinine and blood urea nitrogen all significantly decreased while expressions of Nephrin and Podocin in all treatment groups markedly increased.The treatment also alleviated the pathological damage of kidney tissue to some extent.CONCLUSION Conggui Yishen Capsules are effective remedy for rats with diabetic nephropathy.The mechanism may be related to the up-regulated the expression of Nephrin and Podocin.

关 键 词：糖尿病肾病 苁归益肾胶囊 肾病蛋白 肾小球足细胞裂隙膜蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]