mTOR/p70S6K信号通路在冠心病患者外周血T细胞中的表达及活化  

Study on the activation of m TOR / p70S6K signaling pathway in peripheral T lymphocytes of the CHD patients

作　　者：张艳[1,2] 李志樑[2] 胡春玲[3] 袁建章[3] 

机构地区：[1]广东省妇幼保健院内科 [2]南方医科大学珠江医院心内科,广州市510282 [3]广东省妇幼保健院内科,广州市511400

出　　处：《实用医学杂志》2014年第21期3437-3440,共4页The Journal of Practical Medicine

摘　　要：目的:检测m TOR/p70S6K信号通路在冠心病患者外周血T细胞中的表达及活化情况,探讨其在冠状动脉粥样硬化发生发展中的作用。方法:采用Western blot及RT-PCR法检测冠心病组(AMI、UAP及SAP)及胸痛综合征(CPS)对照组患者外周血T细胞中m TOR及其下游分子p70S6K基因及蛋白表达以及p70S6K磷酸化水平。结果:RT-PCR结果示m TOR、p70S6K基因在AMI、UAP组表达量均显著高于SAP及CPS组(P<0.05);Western blot结果表明,m TOR、p70S6K蛋白表达及p70S6K磷酸化水平在AMI、UAP组均显著高于SAP及CPS组(P<0.05)。结论:冠心病尤其是AMI及UAP组患者T细胞中存在异常活化的m TOR信号,m TOR/p70S6K信号通路在冠状动脉粥样硬化发病中发挥重要作用。Objective To investigate whether mTOR/p70S6K signal was activated in peripheral T lymphocytes of the coronary heart disease （CHD） patients and to investigate its possible role in the pathogenesis of coronary atherosclerosis. Methods The mRNA expressions of mTOR andp70S6K were detected by RT-PCR and protein expressions of mTOR, p70S6K and phosphorylated-p70S6K （p-p70S6K） were detected by Western blot assay in T cells isolated from peripheral blood of the CHD patients （including AMI, UAP, SAP） and the CPS patients. Results Levels of mTOR and p-p70S6K were significantly enhanced in the AMI and UAP groups as compared with those in the SAP and CPS groups （P 〈 0.05, respectively）. The mRNA expressions of mTOR and p70S6K were significantly increased in the AMI and UAP groups as compared with those in the SAP and CPS groups （P 〈 0.05）. Conclusion The mTOR/p70S6K pathway is activated in the peripheral T lymphocytes of CHD patients with AMI and UAP , which may participate in the pathological process of coronary atherosclerosis.

关 键 词：动脉粥样硬化 免疫 哺乳动物雷帕霉素靶蛋白 T淋巴细胞 冠状动脉粥样硬化性疾病 

分 类 号：R541.4[医药卫生—心血管疾病]