透明质酸对人骨关节炎软骨细胞骨桥蛋白和CD44表达的影响  

Effects of hyaluronic acid on osteopontin mRNA and CD44 mRNA expression in human osteoarthritic chondrocytes

作　　者：周斌[1] 张方杰[1,2] 罗伟[3] 高曙光[3] 曾超[3] 熊依林[3] 李宇晟[3] 雷光华[1,2] 

机构地区：[1]中南大学湘雅医院,湖南省长沙市410008 [2]中南大学骨科研究所,湖南省长沙市410008 [3]中南大学湘雅医院骨科,湖南省长沙市410008

出　　处：《中国组织工程研究》2014年第38期6172-6178,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:软骨进行性破坏为晚期骨关节炎的特征性病变,骨关节炎相关因子透明质酸、骨桥蛋白、CD44在骨关节炎软骨中表达增加。目的:通过透明质酸干预体外培养的人膝骨关节炎软骨细胞,探讨透明质酸对人膝骨关节炎软骨细胞CD44与骨桥蛋白表达的影响。方法:将软骨标本进行体外培养获取纯化的软骨细胞,分为3组:空白对照组、透明质酸干预组(100 mg/L)和透明质酸酶干预组(200 mg/L)。培养48 h后,采用Real-time Q PCR检测软骨细胞骨桥蛋白mRNA,CD44mRNA表达水平。用SPSS 17.0统计软件包分析骨桥蛋白mRNA和CD44 mRNA经透明质酸干预前后表达的差异。结果与结论:透明质酸组的骨桥蛋白mRNA表达水平较空白组高,透明质酸酶组的骨桥蛋白mRNA表达水平较空白组低;透明质酸组及透明质酸酶组的CD44 mRNA表达水平均较空白组低。结果提示透明质酸可以上调骨关节炎软骨细胞骨桥蛋白的表达;透明质酸在骨关节炎软骨细胞内对CD44表达的影响具有双相性,其影响结果可能与透明质酸的相对分子质量有关。BACKGROUND： Progressive fracture of the cartilage is considered the characteristic lesion in later osteoarthritis, the expression of osteoarthritis-related factors such as hyaluronic acid, osteopontin and CD44 in osteoarthritic cartilage is increased. OBJECTIVE： To investigate the effect of hyaluronic acid on the expression of osteopontin mRNA and CD44 mRNA of chondrocytes in the in vitro cultured chondrocytes of patients with knee osteoarthritis. METHODS： The cartilage samples obtained from osteoarthritic patients were cultured and purified into acquire chondrocytes in vitro, and the cells were divided into three groups： blank control group, hyaluronic acid （100 mg/mL） group and hyaluronidase （200 mg/mL） group. After 48 hours of cell culture, real-time quantitative polymerase chain reaction assay was used to detect the expression of CD44 mRNA and osteopontin mRNA. The difference of the expression levels before and after the intervention of hyaluronic acid was compared and analyzed using SPSS 17.0 software. RESULTS AND CONCLUSION： Compared with the blank control group, hyalurenic acid （100 mg/mL） upregulated osteopontin mRNA expression in the chondrocytes, hyaluronidase （200 mg/mL） also reduced osteopontin mRNA expression in the chondrocytes. The CD44 mRNA expression in the chondrocytes ofhyaluronic acid （100 mg/mL） group and hyaluronidase （200 mg/mL） group was lower than that in the blank control group Hyaluronic acid can upregulate the expression of the osteopontin mRNA expression in the osteoarthritic chondrocytes; the biphasic effects of hyaluronic acid on CD44 mRNA expression in osteoarthritic chondrocytes might be associated with the molecule weight of hyaluronic acid.

关 键 词：骨关节炎 膝 软骨细胞 骨桥蛋白质 透明质酸 抗原 CD44 组织构建 实时荧光定量RT-PCR 骨桥蛋白 

分 类 号：R318[医药卫生—生物医学工程]