慢性间歇性低压低氧增强丹皮酚舒张大鼠动脉的作用  

Chronic intermittent hypobaric hypoxia enhances vasodilative effects of paeonol on isolated thoracic aorta rings of rats

作　　者：郭赞[1] 宋士军[1] 宋爽[2] 马坤[2] 于雷[2] 宋彦丽[2] 马会杰[1] 张翼[1] 

机构地区：[1]河北医科大学生理学教研室 [2]河北医科大学2008级临床医学专业,河北石家庄050017

出　　处：《中国药理学通报》2014年第11期1574-1579,共6页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2006CB5041006);河北省科技资助计划(No 11246125D);河北省卫生厅资助项目(No 20110042);河北省教育厅项目(No 2011154)

摘　　要：目的研究慢性间歇性低压低氧(CIHH)对丹皮酚舒张大鼠离体胸主动脉环作用的影响,并探讨其可能的机制。方法♂SD大鼠,随机分为CIHH组和常氧对照组。CIHH大鼠给予28 d相当于海拔5 000米高度,每天6 h的CIHH处理;制备胸主动脉环,将动脉环置于浴槽内,恒温灌流、并记录其舒缩活动。结果 CIHH对去甲肾上腺素(NE)和KCl引起的大鼠离体血管收缩活动无影响;CIHH增强了丹皮酚舒张大鼠离体胸主动脉环的作用;在CIHH组,丹皮酚舒张大鼠离体血管的作用可被心得安、格列苯脲和L-NAME部分阻断,丹皮酚抑制NE诱发的细胞内钙和外钙性收缩,而在正常氧组,未出现格列苯脲的阻断效应和对细胞内钙性收缩的抑制效应。吲哚美辛对丹皮酚舒张正常氧和CIHH大鼠血管的效应均无阻断作用。结论 CIHH增强了丹皮酚舒张大鼠离体胸主动脉环的作用,推测其机制是增强了与正常氧相同的作用机制,还通过激活ATP敏感性K+通道和抑制内钙释放而发挥作用。Aim To investigate the effect of chronic intermittent hypobaric hypoxia （ CIHH） on the paeonol induced vasomotion of isolated rat ’ s thoracic aorta rings and its underlying mechanisms. Methods Spra-gue-Dawlay （ SD ） rats were randomly divided into 2 groups： control group （ CON ） and CIHH treatment group （ CIHH） . CIHH rats were exposed to hypoxia in a hypobaric chamber simulating 5 000 m altitude, 6 hours daily for 28 days. CON rats lived in the same en-vironment as CIHH animals except hypoxia. Organ bath technique was used to observe the effect of pae-onol on isolated thoracic aorta rings of rats. Results There were no significant differences of noradrenaline （ NE ）- and KCl-induced contraction in thoracic aorta rings among CIHH and CON rats;CIHH enhanced va-sodilative effects of paeonol on isolated thoracic aorta rings of rats; the vasodilative effects on CIHH rats could be partly decreased by β-receptor blocker prop-ranolol,ATP-sensitive potassium channel （ KATP ） bloc-ker glibenclamide and NO synthase inhibitor L-NAME. Paeonol significantly inhibited NE-induced intracellular and extracellular calcium-dependent contraction in CIHH rats. Paeonol didn ’ t inhibit NE-induced con-traction by intracellular calcium release and its inhibi-tory effect couldn ’ t be blocked by glibenclamide in CON. Vasodilative effects of paeonol couldn ’ t be re-versed by indomethacin, a cyclooxygenase inhibitor, in CIHH and CON rats. Conclusion CIHH significantly enhances vasodilative effects of paeonol on isolated tho-racic aorta rings of rats. Besides promoting the signa-ling pathway of paeonol in CON, CIHH significantly enhances vasodilative effects of paeonol via activating KATP and inhibiting Ca2＋ release from sarcoplasmic re-ticulum.

关 键 词：慢性间歇性低压低氧 丹皮酚 胸主动脉环 血管舒张 ATP敏感钾通道 大鼠 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]