不同强度正弦交变电磁场对大鼠骨生物力学性能的影响  

Effect on different intensity of sinusoidal electromagnetic fields on the biomechanical properties in rats

作　　者：高玉海[1] 陈克明[1] 葛宝丰[1] 周建[1] 马小妮[1] 李少锋[1] 

机构地区：[1]兰州军区兰州总医院骨科研究所,兰州730050

出　　处：《中国骨质疏松杂志》2014年第10期1170-1174,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(No.81270963);甘肃省科技重大专项计划资助项目(No.092NKDA025)

摘　　要：目的研究50 Hz不同强度正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)对大鼠骨生物力学性能的影响。方法 24只6周龄雌性SD大鼠适应性喂养1 w后,随机等分为3组:正常对照组;0.1 mT SEMFs组;0.6 mT SEMFs组。除对照组外,分别给予各SEMFs组3h/d的磁场干预,8w后双能X骨密度仪检测全身骨密度后处死,分离骨组织于AG-X系列台式电子万能试验机检测左侧股骨和椎骨生物力学指标,micro-CT扫描并分析右侧股骨骨微结构形态改变。剥离心、肝、脾、肺、胃、肾、肾上腺和子宫称重,计算器官指数,并做常规病理学检测。结果各组大鼠脏器的器官指数均无明显差异,病理学观察未见异常改变。与对照相比,50Hz 0.1mT正弦交变电磁场组全身骨密度、股骨最大载荷、弹性模量、屈服强度、第4腰椎最大载荷、弹性模量以及micro-CT扫描股骨得出的骨形态参数中相对骨体积、骨小梁数量和厚度均有极显著增加(P<0.01),骨小梁分离度极明显低于对照组(P<0.01);50Hz 0.6mT正弦交变电磁场组与对照相比无统计差异。结论说明50 Hz 0.1 mT电磁场能增加SD大鼠骨密度,改善骨组织微结构,有效提升骨生物力学性能,降低骨质疏松骨折发生的风险。Objective To investigate the effect of the different intensity of 50 Hz sinusoidal electromagnetic fields（SEMFs） on the biomechanical properties in rats.Methods Twenty-four 6-week-old female SD rats were fed for 1 week.They were randomly divided into 3 groups：normal control group,0.1 mT SEMFs group,and 0.6 mT SEMFs group.Except to rats in the control group,rats in other groups were given SEMFs for 3h every day.After 8 weeks,bone mineral density（BMD） of the whole body was measured using dual-energy X-ray absorptiometry.Bilateral femurs and the tibia were collected.The biomechanical properties of the left femurs and the vertebrae were tested using an AG-X multifunctional detector.The microstructure of the right femurs was scanned and analyzed using μ-CT.The heart,the liver,the spleen,the lung,the stomach,the kidney,the adrenal gland,and the uterus were all collected and weighed.The organ index was calculated and the routine pathological detection was performed.Results The organ index of all the organs showed no significant difference,and the pathological observation also showed no abnormal changes.Comparing to those in control group,the whole body BMD,femur maximum load,elastic modulus,yield strength,the fourth lumbar maximum load,elastic modulus,and bone volume,trabecular number and thickness from micro-CT scans increased significantly in 50 Hz 0.1mT SEMFs group（P 0.01）.Trabecular separation in 50 Hz 0.1mT SEMFs group was significantly lower than that in control group（P 0.01）.No statistical difference was found between 50 Hz 0.6mT SEMFs group and control group.Conclusion 50 Hz 0.1 mT SEMFs can effectively increase BMD,improve the bone microstructure organization and the biomechanical properties,and reduce the risk of osteoporotic fractures.

关 键 词：正弦交变电磁场 骨密度 骨生物力学 大鼠 

分 类 号：R244[医药卫生—针灸推拿学] R318[医药卫生—中医临床基础]