六周递增负荷游泳运动过程中小鼠免疫平衡的动态观察——流式细胞仪对Th1/Th2细胞的直接检测  

Effect of Six-Weeks Incremental Swimming Exercise on Immune Balance in Mice——Th1,Th2 cells are assessed by Flow Cytometry

作　　者：谢东北[1] 徐凯星 郝选明[2] 

机构地区：[1]赣南师范学院,江西赣州341000 [2]华南师范大学,广东广州510631

出　　处：《广州体育学院学报》2014年第6期76-80,共5页Journal of Guangzhou Sport University

基　　金：江西省教育厅科技项目(项目编号:GJJ08390)

摘　　要：研究目的:以Th1、Th2细胞为指标,观察递增负荷游泳运动过程中免疫平衡的动态变化。研究方法:雄性8周龄SPF级昆明小鼠进行六周的递增负荷游泳训练,每天一次,每周六天。分别在0周、2周、4周和6周取血,流式细胞术直接测定血液Th1、Th2细胞数量。研究结果;六周递增负荷游泳运动过程中Th1细胞数量第2周明显下降,2周、4周呈上升恢复趋势,6周时基本恢复到正常;Th2细胞运动后2周、4周和6周各组均显著上升。提示在六周的运动过程中,小鼠的体液免疫功能呈增强趋势;在六周的运动周期中,各个采样时点Th1/Th2比值都显著下降。研究结论:六周递增负荷游泳运动过程中,Th1细胞数量的下降,同时Th2细胞增多。Th1/Th2平衡向Th2一侧极化,提示免疫平衡向体液免疫侧漂移。Objection： This research is designed to explore the change of immune balance （Th1/Th2）of mice during six weeks incremental load exercise. Method ：64 male 8 -week old SPE grade KM mice are divided randomly into 8 groups. The experi- mental groups conduct incremental swimming exercise, once a day, and six days a week for 6 weeks. The blood is got respec- tively 0 weeks ,2 weeks, 4 weeks and 6 weeks after exercise. Th1, Th2 cells are calculated by flowcytometry. Result： （ 1 ） The amount of Thl cell droped after exercise, most obvious after 2 weeks, and take on recovery trend in the sixth week. （2） The amount of Th2 cell rise obviously after 2, 4 and 6weeks. （ 3 ） The Th1/Th2 of experimental groups rise obviously in comparision with control group. Conclusion： 1. The number of Thl cells declined and Th2 cells Raised in the six -week incremental swimming exercise. Th1/Th2 balance is broken and polarized to Th2. exercise immunity,

关 键 词：免疫平衡 递增负荷运动 TH1/TH2 小鼠 

分 类 号：G804.32[文化科学—运动人体科学]