聚乳酸-聚乙二醇共聚物/表面接枝复合纤维材料与成骨细胞的黏附、增殖及活性  

Effect of polylactic acid-polyethylene glycol composite fibrous materials on adhesion, multiplication and viability of osteoblasts

作　　者：卢剑[1] 杨振磊[2] 侯任[3] 

机构地区：[1]桂林医学院附属医院脊柱骨病外科,广西壮族自治区桂林市541000 [2]菏泽市立医院关节外科,山东省菏泽市274000 [3]桂林医学院附属医院妇产科,广西壮族自治区桂林市541000

出　　处：《中国组织工程研究》2014年第39期6246-6251,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大量的研究表明,应用于骨组织工程的材料,如羟基磷灰石及高分子聚乳酸等,均对骨缺损的修复起到了一定的作用。但单一的材料在组织相容性及仿生学等特点诸多方面,无法满足骨组织生长的需要,将现有的单一材料进行改性再复合产生新的材料是骨组织工程发展的一个重要方向。目的:通过静电纺丝技术制备共混的聚乳酸-聚乙二醇共聚物/表面接枝羟基磷灰石复合纤维材料,探讨表面接枝羟基磷灰石的引入及其在材料中的含量对成骨细胞在新型材料黏附、增殖和细胞活性的影响。方法:通过调整静电纺丝溶液中聚乳酸-聚乙二醇共聚物和表面接枝羟基磷灰石的4个质量百分数比例,5%/0%,5%/5%,5%/10%,5%/15%,分别制备的4种新型材料(样品1,2,3,4)与小鼠成骨细胞共培养,在特定的时间点,通过细胞染色、碱性磷酸酶检测等实验方法,获得4种不同材料对与之共培养的成骨细胞的相关数据。通过对上述数据进行统计分析,评价新型材料对成骨细胞黏附、增殖能力及细胞活性的影响。结果与结论:在细胞黏附实验中,3组含有表面接枝羟基磷灰石成分的新型材料与不含的材料相比,细胞数量均增长明显,各组细胞增殖情况和碱性磷酸酶活性从高到低依次为样品3组>样品4组>样品2组>样品1组(P<0.05)。结果证实,聚乳酸-聚乙二醇共聚物中引入表面接枝羟基磷灰石后其生物活性得到提高,且新型材料中表面接枝羟基磷灰石含量可影响材料与成骨细胞相容性及细胞活性。BACKGROUND：Recent advances in polylactic acid-polyethylene glycol （PLA-PEG） block copolymer and hydroxyapatite surface-grafted with poly（l-lactide） （HA-g-PLLA）, including their synthesis and preparation, have been approved for application in bone tissue engineering. But they have stil many disadvantages. Combination of HA-g-PLLA and PEG-PLA could show a great potential for development in bone tissue engineering. OBJECTIVE：To investigate the effect of PLA-PEG/HA-g-PLLA composite fibrous material with different content of HA-g-PLLA on adhesion, multiplication and cellviability of osteoblasts. METHODS：Four fibrous materials of 5%PLA-PEG, 5%PLA-PEG/5%HA-g-PLLA, 5%PLA-PEG/10%HA-g-PLLA, 5%PLA-PEG/15%HA-g-PLLA were prepared using electrospinning technique and divided into four experimental groups. Mouse osteoblasts were inoculated on the materials. A statistical analysis was done based on results from celldyeing and alkaline phosphatase detection at certain time points to evaluate the effect of new materials on osteoblast adhesion, proliferation and viability. RESULTS AND CONCLUSION：In the experiment of cellmultiplication and the test of alkaline phosphatase activity, both absorbance values and activity of alkaline phosphatase from high to low were 5%PLA-PEG/10%HA-g-PLLA〉5%PLA-PEG/15%HA-g-PLLA〉5%PLA-PEG/5%HA-g-PLLA〉5%HA-g-PLLA （P〈0.05）. The absorbance value of PLA-PEG/HA-g-PLLA roughly presented a waveform with the increasing addition of HA-g-PLLA. The effect of PLA-PEG/HA-g-PLLA composite fibrous material on adhesion, multiplication and osteogensis of osteoblasts increases with addition of HA-g-PLLA in a certain range.

关 键 词：生物材料 骨生物材料 聚乳酸-聚乙二醇共聚物 表面接枝羟基磷灰石 碱性磷酸酶 聚乳酸 复合材料 生物相容性 黏附 增殖 细胞活性 

分 类 号：R318[医药卫生—生物医学工程]