HCV在中国流行多样性和独特生长史的分析  

Great variety and unique growth history of HCV in China

作　　者：童汪霞[1] 朱建芸[2] 雷姿颖[2] 徐启桓[2] 罗宁[1] 黄小亮 

机构地区：[1]柳州市铁路中心医院南方医科大学附属柳州医院,广西545005 [2]中山大学附属第三医院传染病学教研室 [3]平果县人民医院

出　　处：《中华实验和临床病毒学杂志》2014年第5期336-338,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 探讨中国丙型肝炎患者中HCV的基因型分布类型,并进行HCV在中国流行病学历史分析,进而研究HCV在中国的分子流行病学和进化动力学特征.方法 从423例丙型肝炎患者的血清中提取HCV RNA并进行cDNA反转录,扩增E1和NS5B两个基因区的核苷酸序列,排除不合格及不适合分析的病毒株序列,再对合格序列进行系统发育分析（phylogenetic analysis）.运用BEAST软件中的Bayesian MCMC （Markov Chain Monte Carlo）算法来进行Coalescence分析,通过重构BSPs（Bayesian skyline plots）图回溯HCV的流行病学历史.结果 HCV分离株的基因分型如下：共包括6个基因型,12个基因亚型（1b：65.9％,6a：17.1％,2a：7.4％,3a：3.6％,3b：3.3％,6e：0.76％,1a,1c,2b,2f,4d以及5a共占0.25％）,以及2个新基因型6变异体.所产生的5个BSP曲线图均凸显1993年至2000年这一时间段,中国的HCV感染数量呈现指数增长,随后则陡然下降.结论 证实了HUV在中国多样性的流行现状,反映了HCV不断变化的基因型流行模式;1993年至2000年期间由于“单采血浆”事件的影响,HCV在中国的感染数量经历了一段“指数”增长期.Objective To determine the constantlychanging HCV genotype distribution patterns in China and investigate the HCV epidemic history and evolutionary dynamics.Methods HCV RNA from 423 HCV patients was extracted using Qiagen Viral RNA extraction kit,cDNA was transcripted using RevertAid First Strand cDNA Synthesis kit,and both E1 and NS5B sequences were amplified using RT-PCR followed by fluorescent sequencing.The sequences were analyzed using phylogenetic trees.The evolutionary analyses were performed using the Bayesian MCMC （Markov Chain Monte Carlo） algorithm implemented in the BEAST software and the BSP was reconstructed to form a retrospective picture of the epidemic history of HCV.Results Six HCV genotypes and 12 subtypes were identified （1b：65.9%,6a：17.1%,2a：7.4%,3a：3.6%,3b：3.3%,6e：0.76%,1a,1c,2b,2f,4d 5a：0.25%）,in addition to two novel HCV-6 variants.A period from 1993 to 2000 was highlighted by the five BSPs,during which rapid HCV growth appeared followed by an abrupt slowing.Conclusions A great diversity in HCV was detected,which reflected a constantly changing pattern of HCV genotypes in China over time.They concurrently highlighted fast HCV growth in 1993-2000.This is a period during which HCV infection become explosive in China because of an officially encouraged plasma campaign in the1990s.

关 键 词：肝炎 丙型 贝叶斯定理 流行病学 分子 序列分析 

分 类 号：R512.63[医药卫生—内科学]