A vascular endothelial growth factor activating transcription factor increases the endothelial progenitor cells population and induces therapeutic angiogenesis in a type 1 diabetic mouse with hindlimb ischemia  

作　　者：Diao Yongpeng Lian Lishan Guo Lilong Chen Houzao Chen Yuexin Song Xiaojun Li Yongjun 

机构地区：[1]Department of Vascular Surgery, Peking Union Medical CollegeHospital, Peking Union Medical College, Chinese Academy ofMedical Sciences, Beijing 100730, China [2]National Laboratory of Medical Molecular Biology, Institute of BasicMedical Sciences, Peking Union Medical College, Chinese Academyof Medical Science, Beijing 100730, China

出　　处：《Chinese Medical Journal》2014年第20期3623-3629,共7页中华医学杂志（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No, 81270399）.

摘　　要：Background Therapeutic angiogenesis has been shown to promote blood vessel growth and improve tissue perfusion. Vascular endothelial growth factor （VEGF） plays an important role in angiogenesis. However, it has side effects that limit its therapeutic utility in vivo, especially at high concentrations. This study aimed to investigate whether an intramuscular injection of a genetically engineered zinc finger VEGF-activating transcription factor modulates the endothelial progenitor cells （EPC） and promotes therapeutic angiogenesis in a hindlimb ischemia model with type 1 diabetes. Methods AIIoxan （intravenous injection） was used to induce type I diabetes in C57BL/6 mice （n=58）. The ischemic limb received ZFP-VEGF （125 pg ZFP-VEGF plasmid in 1% poloxamer） or placebo （1% poloxamer） intramuscularly. Mice were sacrificed 3, 5, 10, or 20 days post-injection. Limb blood flow was monitored using laser Doppler perfusion imaging. VEGF mRNA and protein expression were examined using real-time PCR and ELISA, respectively. Capillary density, proliferation, and apoptosis were examined using immunohistochemistry techniques. Flow cytometry was used to detect the EPC population in bone marrow. Two-tailed Student＇s paired t test and repeated-measures analysis of variance were used for statistical analysis. Results ZFP-VEGF increased VEGF mRNA and protein expression at 3 and 10 days post-injection, and increased EPC in bone marrow at day 5 and 20 post-injection compared with controls （P〈0.05）. ZFP-VEGF treatment resulted in better perfusion recovery, a higher capillary density and proliferation, and less apoptosis compared with controls （P〈0.05）. Conclusions Intramuscular ZFP-VEGF injection promotes therapeutic angiogenesis in an ischemic hindlimb model with type 1 diabetes. This might be due to the effects of VEGF on cell survival and EPC recruitment.Background Therapeutic angiogenesis has been shown to promote blood vessel growth and improve tissue perfusion. Vascular endothelial growth factor （VEGF） plays an important role in angiogenesis. However, it has side effects that limit its therapeutic utility in vivo, especially at high concentrations. This study aimed to investigate whether an intramuscular injection of a genetically engineered zinc finger VEGF-activating transcription factor modulates the endothelial progenitor cells （EPC） and promotes therapeutic angiogenesis in a hindlimb ischemia model with type 1 diabetes. Methods AIIoxan （intravenous injection） was used to induce type I diabetes in C57BL/6 mice （n=58）. The ischemic limb received ZFP-VEGF （125 pg ZFP-VEGF plasmid in 1% poloxamer） or placebo （1% poloxamer） intramuscularly. Mice were sacrificed 3, 5, 10, or 20 days post-injection. Limb blood flow was monitored using laser Doppler perfusion imaging. VEGF mRNA and protein expression were examined using real-time PCR and ELISA, respectively. Capillary density, proliferation, and apoptosis were examined using immunohistochemistry techniques. Flow cytometry was used to detect the EPC population in bone marrow. Two-tailed Student＇s paired t test and repeated-measures analysis of variance were used for statistical analysis. Results ZFP-VEGF increased VEGF mRNA and protein expression at 3 and 10 days post-injection, and increased EPC in bone marrow at day 5 and 20 post-injection compared with controls （P〈0.05）. ZFP-VEGF treatment resulted in better perfusion recovery, a higher capillary density and proliferation, and less apoptosis compared with controls （P〈0.05）. Conclusions Intramuscular ZFP-VEGF injection promotes therapeutic angiogenesis in an ischemic hindlimb model with type 1 diabetes. This might be due to the effects of VEGF on cell survival and EPC recruitment.

关 键 词：diabetes endothelial progenitor cell zinc finger protein vascular endothelial growth factor angiogenesis 

分 类 号：R587.1[医药卫生—内分泌]