拟南芥AtTPS1基因的生物信息学分析与功能预测  

Bioinformatics Analysis and Function Prediction of At TPS1 Gene in Arabidopsis thaliana

作　　者：陆玉建[1,2,3] 张伟[4] 张韩杰[1] 韩文瑜[3] 沈志强[2,5] 

机构地区：[1]滨州学院生命科学系/山东省黄河三角洲野生植物资源开发利用工程技术研究中心,山东滨州256603 [2]山东省滨州畜牧兽医研究院博士后科研工作站,山东滨州256600 [3]吉林大学博士后科研流动站,吉林长春130062 [4]信阳师范学院生命科学学院,河南信阳464000 [5]山东省滨州畜牧兽医研究院,山东滨州256600

出　　处：《生物技术》2014年第5期34-39,共6页Biotechnology

基　　金：山东省自然科学基金项目("耐盐高蛋白小麦的分子选育";No.ZR2012CL14);滨州学院博士基金项目("At ZW10功能的研究";No.2010Y08)资助

摘　　要：目的:对At TPS1基因及其编码产物的结构、表达定位、分子进化、蛋白互作等进行预测和分析。方法:利用在线网络生物信息学相关数据库和分析软件对目的蛋白的同源性、保守结构域、疏水性、跨膜区进行分析;并对基因的表达情况、蛋白质的亚细胞定位、蛋白质间的相互作用进行预测。结果:At TPS1表现出较强的亲水性,在多肽链的内部可能存在跨膜结构和保守结构域。在拟南芥的不同组织和器官都可检测到At TPS1基因的表达,尤其在茎顶端分生组织、种子和花中表达强烈。At TPS1在叶绿体中分布最为广泛,其次是线粒体和细胞核。至少有四类蛋白与At TPS1之间存在较为强烈的相互作用。结论:生物信息学分析结果表明,At TPS1功能复杂,很可能在细胞分裂、胚胎和花发育、种子萌发、光合作用、物质代谢等过程中充当重要的角色。Objective： The structure,expression,localization,molecular evolution,protein- protein interaction of At TPS1 gene and its encoded product were predicted and analyzed. Method： Protein homology,conserved domains,hydrophobicity,transmembrane regions,gene expression,protein localization,protein- protein interaction were predicted and analyzed,adopting the online network relevant databases and bioinformatics softwares. Result： At TPS1 had strong hydrophilicity and significant hydrophobic regions within the polypeptide chain,which was likely to form transmembrane structures in these areas. The expression of At TPS1 gene could be detected in the different tissues and organs of Arabidopsis thaliana. Especially in the shoot apical meristem,seeds and flowers,the gene strongly expressed. At TPS1 was most widely distributed in the chloroplasts,mitochondria and nuclei followed. The more intense interactions existed between at least four kinds of proteins and At TPS1. Conclusion： Bioinformatics analysis showed that the function of At TPS1 is quite complex,it is possible to play important roles in cell division,embryogenesis,flower development,seed germination,photosynthesis,metabolism and other processes.

关 键 词：生物信息学 拟南芥 At TPS1基因 海藻糖 胁迫 

分 类 号：Q786[生物学—分子生物学]