绒山羊70d胚胎与新生羊羔体侧部皮肤miRNA的比较分析  

Analysis of miRNAs between 70 Days Fetal and Lamb Skin

作　　者：郝玉[1] 尹俊[1] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《生物技术》2014年第5期71-76,共6页Biotechnology

基　　金：国家自然科学基金项目("BMP信号在绒山羊绒毛再生周期波中调节干细胞激活的研究";No.30960244);内蒙古自然科学基金重大项目("雪兔毛色季节性变化功能基因组学的研究;No.2013ZD06)资助

摘　　要：目的:最近发现miRNA对哺乳动物毛囊的发育和维持具有重要作用,研究miRNA在绒山羊次级毛囊发育中的作用,可以为人工调节绒毛生长和提高羊绒产量提供新的线索。方法:应用Exiqon miRNA8.1表达谱芯片对70d胎儿皮肤和出生2w羊羔体侧部皮肤组织差异表达的miRNA基因进行筛查,找出绒山羊皮肤发育不同阶段差异表达的miRNA。结果:绒山羊70d胎儿皮肤中相对于出生2w羊羔体侧部皮肤上调的miRNA 4个。绒山羊70d胎儿皮肤中相对于出生2w羊羔体侧部皮肤下调的miRNA 64个。结论:绒山羊70d胚胎皮肤中上调的miRNA远远少于出生后羊羔皮肤上调miRNAs,说明miRNA在皮肤毛囊不同发育阶段中发挥重要作用。Objective： Recently,the miRNAs were found that plays an important role on of the hair follicle development and the maintenance. Study the role of the miRNAs during secondary hair follicles development of the cashmere goat,in order to provide new clues for regulating the cashmere growth and improving the cashmere production. Method： The differentially expressed miRNAs are screening between skin of 70 d fetal and two weeks lamb by Exiqon miRNA8. 1 expression profile chip. Result： There are 4 miRNAs up- regulated in Cashmere goats 70 days fetal skin compared with lambs. 64 miRNAs are down- regulated in 70 days fetal skin compared with lambs. Conclusion： This result indicate that the miRNAs play an important roles in different development stages of cashmere goat skin.

关 键 词：绒山羊 MIRNA芯片 次级毛囊 表达 发育 

分 类 号：S818.9[农业科学—畜牧学]