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机构地区:[1]运城市水务局水产站,山西运城044000 [2]山西省水产技术推广站,山西太原030002 [3]山西农业大学动物科技学院,山西太谷030801
出 处:《生物技术》2014年第5期76-79,共4页Biotechnology
摘 要:目的:了解异齿亚纲贝类的分类地位。方法:从NCBI数据库下载了异齿亚纲贝类中25个不同种的18S rRNA序列,用MEGA 5.0软件进行序列比对,用3种不同的算法构建出异齿亚纲的系统发育树。结果:显示缝栖蛤科最早与其它的7科分开,发生了较大的遗传分化,其余类群以较高的支持率聚在一起。在NJ树与ML树中蛤蜊科与海螂目的海螂科、蓝蛤科、船蛆科和海笋科聚在一起形成一个大支,而在MP树中蛤蜊科又与帘蛤科和蹄蛤科聚为一支。结论:25个不同种并未按照这两个目聚为清晰明确的两个大支,而是相互之间有嵌套。Objective: To futher understanding the classification status clearly that the orders and families under Heterodonta. Method: The18 S RNA sequences including the 25 species in this subclass shells were obtained from National Center of Biotechnology Information( NCBI),and the 3 kinds of phylogenetic tree of Heterodonta by MEGA 5. 0 software were constructed based on different algorithms,and then analyzed. Result: Hiatellidae can be separated to other 7 families earlily,which is caused by the larger genetic differentiation. However,other taxa can be gathered into a cluster according to higher support rate. NJ and ML trees supports the placement of orders Mactridae,Corbulidae,Teredinidae and Pholadidae in one clade. But MP tree supports the placement of orders Mactridae,Veneroida and Ungulinidae in one clade. Conclusion: 25 different species could not be divided into clearly two large branches,but were nested with each other.
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