脱氧胆酰酪氨酸热敏凝胶剂对小型猪阴道黏膜刺激的病理学评价  

Pathological evaluation of the deoxycholyltyosin thermoreversible gel in the mini-pig vagina

作　　者：田芳[1] 王玉柱[1] 职瑞娜 夏敏杰[1] 温成丽[1,2] 王忠辉[3] 周新初[3] 李卫华[1] 

机构地区：[1]上海市计划生育科学研究所,国家计划生育药具重点实验室,上海200032 [2]复旦大学公共卫生学院 [3]上海交通大学农业与生物学院

出　　处：《毒理学杂志》2014年第5期342-346,共5页Journal of Toxicology

基　　金：国家科技重大专项(2012ZX10001007-009-003;2013ZX10001006-003-002);十二五国家科技支撑计划(2012BAI31B04);国家自然科学基金青年基金(81100459);上海市科委项目(13431900800);上海市人口和计划生育委员会(2012JG03)

摘　　要：目的评价脱氧胆酰酪氨酸热敏凝胶剂(DCT gel)的小型猪阴道黏膜刺激性。方法将9只雌性小型猪随机分为空白胶组、DCT-gel组和市售壬苯醇醚凝胶(N-9 jelly)组,阴道给予5 ml凝胶,每日1次,连续给药7 d,末次给药72 h后处死小型猪,取阴道组织,观察阴道黏膜组织病理学改变;免疫组化法检测阴道黏膜内CD45的表达。结果小型猪阴道黏膜组织HE染色显示DCT gel对黏膜上皮层、固有层、血管及周围组织无明显损伤(5.5±2.7),与空白胶组相比差异无统计学意义(3.3±1.6),远低于N-9 jelly组(20.5±3.5,P<0.05);与空白胶组相比,DCT gel组的阴道黏膜CD45表达差异无统计学意义(P>0.05),N-9 jelly组CD45表达显著增加(P<0.05)。结论 DCT gel对小型猪阴道黏膜刺激性较小,作为阴道外用杀微生物剂具有一定的应用前景。Objective To evaluate the vaginal irritation of the Deoxycholyltyosin Thermoreversible Gel（ DCT gel） in the mini-pig model. Methods Nine female mini-pigs were randomly divided into control group,DCT-gel group and commercially nonoxynol gel（ N-9jelly） group,administrated 5ml gel vaginally once daily for 7 days. Mini-pigs were sacrificed after 72 h at the last administration. The vaginal tissues were harvested and vaginal mucosa histopathological changes were observed. The CD45 expression was semi-quantitated by immunohistochemical assay in the vaginal mucosa. Results Vaginal mucosa HE staining showed it had no obvious damage（ 5. 5 ± 2. 7） on mucosal epithelium,lamina propria,blood vessels and surrounding tissue in DCT gel group. It had no significant difference（ 3. 3 ± 1. 6）compared to the control group,but it has significant difference（ 20. 5 ± 3. 5,P〈0. 05） compared to N-9 jelly group. Compared to the control group,CD45 expression of vaginal mucosa in DCT gel group was not significantly different（ P〉0. 05）,and significantly increased in N-9 jelly group（ P〈0. 05）. Conclusion Vaginal irritation of DCT gel on min-pigs is mild. DCT gel has a good prospect as the topical vaginal microbicide.

关 键 词：脱氧胆酰酪氨酸 小型猪 阴道黏膜 CD45 

分 类 号：R994.1[医药卫生—毒理学]