睡眠呼吸暂停相关性高血压患者脂质代谢紊乱与血清补体C1q肿瘤坏死因子相关蛋白1多态性相关  

Association of C1q tumor necrosis factor-related protein 1 genetic variation with dyslipidemia in sleep apnea-related hypertension

作　　者：孟静[1] 李南方[1] 姚晓光[1] 邵亮[1] 张菊红[1] 周玲[1] 张晶晶[1] 孔剑琼[1] 

机构地区：[1]新疆维吾尔自治区人民医院高血压中心,新疆高血压研究所,新疆乌鲁木齐830001

出　　处：《中华高血压杂志》2014年第9期865-870,共6页Chinese Journal of Hypertension

基　　金：国家自然科学基金资助项目(81260017)

摘　　要：目的探讨睡眠呼吸暂停相关性高血压患者脂质代谢紊乱与血清补体C1q肿瘤坏死因子相关蛋白1(CTRP1)基因变异的相关性。方法选取2010年1月1日至12月31日在新疆维吾尔自治区人民医院就诊的经多导睡眠监测的高血压患者838例[男615例,年龄(45.7±9.6)岁,女223例,年龄(49.6±9.8)岁],其中阻塞性睡眠呼吸暂停综合征(OSAS)组[呼吸暂停低通气指数(AHI)≥5次/h,即睡眠呼吸暂停相关性高血压患者]613例,非OSAS组(AHI<5次/h)225例。测序筛查CTRP1基因功能区的变异位点,应用Taqman-PCR技术进行基因型鉴定后分析结果。结果在96例重度OSAS的患者中进行CTRP1基因功能区测序,发现8个变异位点。经Taqman-PCR分型成功共3个变异位点:rs4789922、rs11077409和rs76683473。OSAS组与非OSAS组的CTRP1基因变异位点的基因型频率分布差异无统计学意义。OSAS组中,与脂质代谢正常者比较,脂质代谢紊乱者的低高密度脂蛋白胆固醇(HDL-C)(68.4%比54.3%)和高三酰甘油者(75.0%比54.3%)rs76683473变异位点的GG基因型频率分布较高(均P<0.05),而高总胆固醇和高低密度脂蛋白胆固醇(LDL-C)差异无统计学意义(均P>0.05)。Logistic回归分析显示,校正体质量指数、空腹血糖等影响因素后,OSAS患者中rs76683473变异位点的GG基因型是脂质代谢紊乱(OR=1.96)、低HDL-C(OR=1.52)和高三酰甘油(OR=1.83)的危险因素。结论OSAS患者中,CTRP1基因变异与三酰甘油升高、HDL-C降低有关。Objective To investigate the association of Clq tumor necrosis factor-related protein 1 （CTRP1） genetic variation and dyslipidemia in sleep apnea-related hypertension. Methods A toal of 838 subjects diagnosed with clinical symptoms of obstructive sleep apnea syndrome （OSAS） in the People＇s Hospital of Xinjiang Uygur A.utono- mous Region between January 1 and December 31 of the year 2010 were recruited. Among them, 615 were males with an average age of （45.7±9.6） years and 223 females with an average age of （49.6±9.8） years. All subjects were classified into the OSAS group （n= 613,defined as apnea-hypopnea index ≥5/h} and non-OSAS group （n： 225 ,defined as apnea-hypopnea index〈5/h）. Functional loci of CTRPI gene were sequenced, and genotyping was conducted by Taqman-PCR method. Results A total of 8 functional loci of CTRP1 gene were found in 96 patients with severe OSAS, and only three loci （rs4789922, rs11077409 and rs76683473} were successfully genotyped by Taqman-PCR method. No statistical significance was observed for the genotype frequencies of aforementioned three variants between the OSAS group and non-OSAS group. In the OSAS group, rs76683473-GG genotype was over- represented in dyslipidemia patients with low high-density lipoprotein cholesterol （HDL-C） （68.4% vs 54.3 %） and high triglyceride{TG} {75.0% vs 54.3 %） than patients with no n-dyslipidemia, respectively （both P〈0.05）, while

关 键 词：阻塞性睡眠呼吸暂停 高血压 脂质代谢紊乱 血清补体C1q肿瘤坏死因子相关蛋白1 

分 类 号：R544.1[医药卫生—心血管疾病]