抑制T细胞增殖的抗CD45单克隆抗体的建立  

Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation

作　　者：苏泽红[1] 李亚林[2] 谭翔文[2] 余坚[2] 刘鑫[2] 袁月亲 练高建[2] 

机构地区：[1]南华大学药学与生物科学学院,湖南衡阳421001 [2]南华大学实验动物学部,湖南衡阳421001

出　　处：《中国实验动物学报》2014年第5期35-38,I0003,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：湖南省科技厅项目(2013TT2007;2014TT2012);湖南省教育厅优秀青年项目(13B102);湖南省自然基金(13JJ3081)资助

摘　　要：目的寻找能调节T细胞功能的相关分子,进行与T细胞介导的自身免疫性疾病相关的研究。方法从BALB/c小鼠骨髓中收集树突状细胞,免疫Wistar大鼠,进行细胞融合,建立杂交瘤细胞系。筛选得到很多株能调节T细胞功能的杂交瘤细胞系,对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果显示其目标分子是CD45,同时增殖实验结果显示该抗体能显著抑制T细胞增殖反应。结论抗CD45单克隆抗体能有效抑制T细胞增殖,有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media-ted autoimmune diseases.Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines.Many hybridoma cell lines which could modulate T-cell functions were obtained.One of the cell lines, most actively inhibiting T-cell proliferation, was further studied.Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays.Conclusions Our re-sults indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the pre-vention and treatment of T-cell mediated autoimmune diseases in the future.

关 键 词：CD45 单克隆抗体 抑制 T细胞增殖 树突状细胞 小鼠 大鼠 

分 类 号：Q95-33[生物学—动物学]