四氧嘧啶诱导的实验性高血糖小鼠模型建立及其稳定性  

Establishment and stability of a hyperglycemic mouse model induced by alloxan

作　　者：陈琳[1] 乐凯[1] 茹琴[1] 田香[1] 熊琪[1] 马宝苗[1] 刘璐[1] 吴日辉 邢俊俏 王宁 张琨 赵小伟 陈卫 何丽 欧阳康乐 司远仁 李超英[1,4] 

机构地区：[1]江汉大学武汉生物医学研究院,湖北武汉430056 [2]福格森(武汉)生物科技股份有限公司,湖北武汉430050 [3]湖北省中山医院,湖北武汉430032 [4]汉济生物科技(武汉)有限公司汉济生物医药研究院,湖北武汉430075

出　　处：《中国比较医学杂志》2014年第10期32-38,共7页Chinese Journal of Comparative Medicine

基　　金：湖北省教育厅科学技术研究计划指导性项目(编号:B2013144)

摘　　要：目的：探讨剂量、溶媒等主要影响因素对四氧嘧啶致实验性高血糖小鼠模型建立及稳定性的影响。方法以SPF级昆明种小鼠为实验对象，连续6周观察四氧嘧啶剂量、溶媒的不同组合对造模成功率、存活率、体重、空腹血糖值、血糖曲线下面积的稳定性及血清胰岛素水平的影响。结果160mg/kg体重四氧嘧啶（pH值为4.5的柠檬酸钠缓冲液溶解），单次腹腔注射，可获得成模率70％、存活率75％、空腹血糖（15～20mmol/L）及血糖曲线下面积（55～65mmol/L）持续6周稳定，血清胰岛素水平（21mIU/L）显著降低但不丧失的实验性高血糖小鼠模型。结论本实验充分考虑剂量、溶媒等主要影响因素对四氧嘧啶致实验性高血糖小鼠模型的影响，并进行6周的模型稳定性动态观察，筛选出理想的造模条件，可用于实验性糖尿病的基础研究及辅助降血糖的功能研究。Objective To explore the influence of drug dosage, solvent and other main influencing factors on the successful establishment of alloxan-induced hyperglycemia mouse model and the effect on the stability of this model. Methods 160 6-8-week-old Kunming mice ofSPF grade, （male：female=1：1） were used in this study.The influences of different dosages of alloxan and solvent combinations on the successful establishment rate of the model, survival rate, body weight, fasting blood glucose, blood glucose area under curve, serum insulin level and their stabilities were dynamically observed for six weeks.Results By single intraperitoneal injection of 160 mg/kg bw alloxan （ pH 4.5 citrate sodium as solvent） , we were able to obtain a stable experimental hyperglycemic mouse model with higher levels of successful establishment rate （70%）, survival rate （75%）, fasting blood glucose （15-20 mmol/L）, glucose area under the curve （55-65 mmol/L） and a lower but not loss of serum insulin levels （21 mIU/L）.Conclusions In the present study we have carefully considered the influence of main factors such as drug dosages, solvent, etc., on the alloxan-induced experimental hyperglycemic mouse model, and successfully established this model after 6-week period observation of its stability.This model may provide a useful tool in the research of experimental diabetes and hypoglycemic functional studies.

关 键 词：四氧嘧啶 实验性高血糖小鼠模型 剂量 溶媒 稳定性 

分 类 号：R33[医药卫生—人体生理学]