达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用  

Improving effects of dammarane sapogenins on sleep interruption-induced learning and memory impairment in mice

作　　者：卜兰兰[1,2] 石哲[2] 武宏伟[2] 卢聪[2] 王克柱[2] 李莹辉[3] 曲丽娜[3] 刘新民[1,4] 

机构地区：[1]湖南中医药大学病理学教研室,湖南长沙410208 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中国航天员中心航天医学基础与应用国家重点实验室,北京100094 [4]湖南省实验动物中心湖南省药物安全评价研究中心,湖南长沙410331

出　　处：《中国比较医学杂志》2014年第10期48-53,66,共7页Chinese Journal of Comparative Medicine

基　　金：国际科技合作专项-人参益智药效与基因/蛋白表达谱关联规律合作研究(编号:2011DFA32730);全军医学科技“十二五”科研项目(编号:BWS11J052);极限环境认知损伤保护药临床前药效评价技术体系-国家科技重大专项课题(编号:2012ZX09J12201);湖南中医药大学病理学与病理生理学重点学科(2012)

摘　　要：目的研究达玛烷苷元(dammarane sapogenins,DS-1226)对睡眠干扰(sleep interruption,SI)致认知障碍小鼠学习记忆能力的影响。方法将ICR小鼠随机分为对照组,模型组,DS-1226低、中、高三个剂量组。用改良滚筒法对小鼠进行睡眠干扰,同时灌胃给药15 d后,用自主活动仪检测自主活动,水迷宫、避暗实验检测学习记忆能力。结果与模型组比较,DS-1226各给药组总路程、平均速度、运动总时间均增加。模型组水迷宫定位航行D5、D6潜伏期增加;与模型组和低剂量组比较,DS-1226中剂量组D6潜伏期降低,高剂量组D1-D6潜伏期降低;与中剂量组比较,DS-1226高剂量组D1-D5潜伏期降低。模型组水迷宫空间探索目标象限穿台次数及游程比率均低于对照组;DS-1226高剂量组目标象限穿台次数、目标象限游程比率及时间比率均高于模型组,目标象限穿台次数高于中剂量组,目标象限游程比率高于低剂量组。模型组避暗学习错误次数、暗室路程、暗室时间、暗室静息时间增加;与模型组比较,DS-1226低、中剂量组错误次数、暗室路程减少,高剂量组错误次数、暗室时间、暗室路程、暗室静息时间减少,明室时间增加。结论水迷宫和避暗实验结果表明DS-1226给药对SI所致小鼠学习记忆障碍有改善作用,且有剂量相关性。Objective To study the effects of dammarane sapogenins （ DS-1226 ） on sleep interruption-induced learning and memory impairment in mice.Methods 130 SPF healthy 5 -6-week old male ICR mice were randomly divided into control, model, DS-1226 low dose, DS-1226 medium dose and DS-1226 high dose groups.The behavioral alterations in open field （OF）, Morris water maze （MWM） and step-through （ST） tests were detected at 15 days after rotating drum-induced sleep interruption （ SI） .Results The total distance, movement speed, total duration of movement were increased in OF test （ P〈0.05, vs.the model group） after treatment.The latency of place navigation was increased from day 5 in the MWM test after 15 d sleep interruption, and the number of crossing in the target quadrant and the percent distance in target quadrant were decreased after 15 d sleep interruption （ P 〈0.05, vs.the control group）, while the latency of place navigation was decreased, and the percent distance in target quadrant and percent time in target quadrant after high dose DS-1226 oral administration （ P〈0.05, vs.the model group） were increased.Error times, distance in dark chamber, time in dark chamber and immobility time in dark chamber were increased in training of step through test （ P〈0.05, vs.the control group）;while these indexes were decreased after DS-1226 oral administration （ P〈0.05, vs.the model group） .But there was no significant difference in the step through testing course.Conclusions The results show that orally administrated DS-1226 can ameliorate SI-induced learning and memory impairment, and there is a significant dosage-effect relationship.

关 键 词：DS-1226 滚筒 睡眠干扰 ICR小鼠 自主活动 水迷宫 避暗 

分 类 号：R33[医药卫生—人体生理学]