慢性低氧大鼠TRPC1表达与肺动脉收缩变化时间曲线关系  

Relationship of time-course curve between the expression of TRPC1 and vascular tone of pulmonary arteries in chronic hypoxia pulmonary hypertension rats

作　　者：穆云萍[1] 焦海霞[1] 朱壮丽[1] 戴耄 黄秋虹[1] 王瑞幸[1] 林默君[1] 

机构地区：[1]福建医科大学基础医学院生理学与病理生理学系/心血管科学研究室,福建福州350108

出　　处：《中国药理学通报》2014年第12期1667-1671,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31171104);福建医科大学重大科研课题资助项目(No 09ZD010)

摘　　要：目的探讨慢性低氧（chronichypoxia，CH）上调TR—PCI表达和SOCE介导肺动脉（pulmonaryarteris，PAs）收缩的时间曲线。方法清洁级6SD大鼠，常压低氧（氧分压为9．5％～10．5％）饲养，观察在不同时问点CH对TRPCI表达量和SOCE介导的PAs收缩的影响，描记其时问依赖性曲线。结果①CH上调平均右心室收缩压（mRVSP），低氧1d压力增高明显，7d达到最大值，21d后达到稳定；右心室质量指数（RVMI）低氧3d后开始增高，此后一直呈持续增高状态，直到21d达到稳定；②半定量RT—PCR检测显示，CH上调TRPCImRNA的表达量，低氧1d上调明显，3d达到最大值一直维持到7d，21d后达到稳定；③CH上调SOCE介导的PAs收缩，低氧3d开始上调，7d达到最大值，21d后达到稳定。结论CH早期TRPCI／SOCE明显上调，两者的时问曲线具有相关性，表明TRPCI和SOCE的上调在慢性低氧致肺高压的机制中发挥着重要作用。Aim To evaluate the time-course curve of expression of TRPC1 and vascular tone of pulmonary arteries（PAs） mediated by SOCE in chronic hypoxiapulmonary hyperte- nsion rats. Methods Both tension of PA rings and expression of TRPC1 were tested in CH exposure （ 10. 0 % ＋ O. 5 % partial pressure of oxygen ）induced pulmonary hypertensive （PH） rats, and the time-course curve（ detected respectively in CH 1,3,5, 7,14,21 d） was traced. Results ①CH could up- regulate the mean right ventricular pressure （mRVSP） , which was increased significantly on ld, and reached the maximum on 7d; right ventricular weight index （RV- MI） began increase on 3d, and kept rising; ② semi-quantitative reverse transcription polyme- rase chain reaction （RT-PCR） was performed to detect the expression of TRPC1 in PAs. The expression of TRPC1 increased significantly on l d, and reached the maxi- mum on 3d; ③CH could up-regulate the vascular toneof PAs mediated by SOCE, which was increased signif- icantly on 3d, and reached the maximum on 7d. Con- clusions TRPC1/SOCE increases significantly in the early days of CH, and the time-course curve of the two has correlation, which reflects the important role of the upregulation of TRPC1/SOCE in the process of chronic hypoxia pulmonary hypertension.

关 键 词：慢性低氧 肺高压 肺动脉 经典瞬时感受器电位 钙池操纵性钙内流 时间曲线 相关性 

分 类 号：R332[医药卫生—人体生理学] R322.121[医药卫生—基础医学]