冰片对鼻咽癌细胞容积敏感性氯通道的激活作用  

Borneol activated volume-sensitive chloride channels in poorly differentiated nasopharyngeal carcinoma cells

作　　者：孟龙[1] 王海波[2] 邓志钦[2] 汪源[2] 伍嘉宝 赖周毅 吕瑞玲[2] 孙晓雪[2] 朱林燕[2] 陈丽新[2] 王立伟[1] 

机构地区：[1]暨南大学医学院生理学系,广东广州510632 [2]暨南大学医学院药理学系,广东广州510632

出　　处：《中国药理学通报》2014年第12期1671-1676,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273539;81173064);教育部基金项目(No 20124401110009);广州市科技计划基金项目(No 2013J450015);东莞科技计划基金项目(No2011108102006)

摘　　要：目的探讨冰片对低分化鼻咽癌(CNE-2Z)细胞氯通道的激活作用及对细胞容积的影响。方法采用全细胞膜片钳技术记录冰片激活CNE-2Z细胞膜电流,分析其电流特性;用活细胞图像法观察并测量冰片对细胞体积的影响。结果细胞外灌流冰片(20μmol·L-1)诱导CNE-2Z细胞膜氯通道开放,产生氯电流,该电流有明显外向优势,无电压及时间依赖性失活,其翻转电位接近氯离子平衡电位,该电流可被氯通道阻断剂tamoxifen抑制,细胞外灌流47%高渗溶液完全抑制该电流。细胞外灌流冰片30 min,细胞体积缩小约9.4%,该作用可被tamoxifen完全抑制。结论冰片可以激活低分化鼻咽癌细胞容积敏感性氯通道,导致细胞体积缩小,氯通道在冰片引起细胞体积缩小中起着重要作用。Aim To clarify the effect of Borneol on the chloride channels and cell volume in poorly differentia-ted nasopharyngeal carcinoma CNE-2Z cells.Methods The technique of whole-cell patch clamp was used to detect the chloride currents and analyze the character-istics of the currents in CNE-2Z cells.The volume changes caused by Borneol were measured by the meth-od of time-lapse live cell imaging.Results The chlo-ride currents were induced by extracellular application of Borneol （20 μmol·L ^-1 ）isotonic condition.The currents showed a characteristic of outward rectification and did not show voltage-dependent or time-dependent inactivation.The reversal potential of the currents was＆amp;nbsp;close to the CI-equilibrium potential. The currents were inhibited by the chloride channel blocker tamox-ifen.The currents were also inhibited by 47% hyper-tonic solution.Borneol decreased the cell volume by 9.4% in 30 min.Tamoxifen completely inhibited the Borneol-induced cell volume decrease.Conclusion Borneol can activate volume-sensitive chloride channels and induce volume decrease in CNE-2Z cells.Chloride channels play a pivotal role in the process of volume decrease caused by Borneol.

关 键 词：冰片 氯通道 氯通道阻断剂 癌细胞 膜片钳技术 细胞体积 

分 类 号：R282.71[医药卫生—中药学] R329.24[医药卫生—中医学]