丙泊酚对癫痫大鼠学习记忆及海马TLR4表达的影响  

Effects of propofol on learning and memorizing ability and hippocampus TLR4 in epileptic rats

作　　者：宋炜[1] 蔡鹏[1] 杜雷[1] 王志达[1] 许志强[1] 张咏梅[1] 

机构地区：[1]徐州医学院麻醉学院,江苏徐州221004

出　　处：《中国药理学通报》2014年第12期1725-1729,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81171041);江苏省自然科学基金资助项目(No BK2011197);江苏省高校自然科学研究重大项目(No 13KJA320001);江苏省高等学校大学生实践创新训练计划项目(No 201310313035Y)

摘　　要：目的探讨丙泊酚对癫痫大鼠学习记忆的影响,以及与苯巴比妥钠联合用药对癫痫大鼠学习记忆的影响。方法36只大鼠均制备癫痫模型,随机分为癫痫模型组(EP)、生理盐水+癫痫组(NS)、脂肪乳+癫痫组(LE)、苯巴比妥钠+癫痫组(PB)组、丙泊酚+癫痫组(Prof)以及苯巴比妥钠与丙泊酚联合用药+癫痫组,每组6只。对每只大鼠进行Morris水迷宫行为测试,评价其学习记忆能力;用酶联免疫吸附方法测定海马TLR4蛋白表达量。结果与癫痫模型组相比,给予生理盐水或脂肪乳对癫痫大鼠学习记忆能力及海马TLR4蛋白表达量均无明显影响(P>0.05)。给予丙泊酚后可使癫痫大鼠潜伏期明显延长,平台停留期缩短,TLR4蛋白表达明显增高(P<0.05)。给予苯巴比妥钠后可使癫痫大鼠平台停留期缩短,TLR4蛋白表达明显增高(P<0.05),但潜伏期无明显差异(P>0.05)。联合用药组与苯巴比妥钠组对比,潜伏期明显延长,平台停留期缩短,TLR4蛋白表达明显升高(P<0.05)。联合用药组与丙泊酚组对比,平台停留期缩短,TLR4蛋白表达明显增高(P<0.05),潜伏期无明显差异(P>0.05)。结论丙泊酚对癫痫大鼠学习和记忆功能均有损害;苯巴比妥钠对癫痫大鼠的学习能力没有明显影响,对其记忆功能有损害;丙泊酚与苯巴比妥钠联合用药对癫痫大鼠的学习和记忆功能均有损害。海马TLR4可能参与了丙泊酚与苯巴比妥钠对癫痫大鼠学习和记忆的影响过程。Aim To explore the effects of propofol on learning and memorizing ability and the effects of com-bination of propofol and phenobarbital sodium on epi-leptic rats.Methods Thirty-six epileptic rats were di-vided into epileptic model group （EP）,normal saline group （NS）,lipid emulsion ＋epileptic group （LE）, phenobarbital sodium ＋epileptic group （PB）,propofol＋epileptic pattern （Prof）,and combination of propo-fol and phenobarbital sodium ＋epileptic group.Each group had 6 rats.Tests of Morris water maze were giv-en to the rats to evaluate their learning and memorizing ability.The protein expression of toll-like receptor 4 （TLR4 ）was examined by ELISA.Results There were no effects of saline and lipid emulsion on learning and memorizing ability and the expression of TLR4 pro-tein in hippocampus in epileptic rats （P〉0.05 ）. Propofol could increase the incubation period in epilep-tic rats obviously,shorten the plateau period,and in-crease the expression of TLR4 protein in hippocampus （P〈0.05 ）.Phenobarbital sodium could shorten the plateau period in epileptic rats,and increase the ex-pression of TLR4 protein in hippocampus （P〈0.05）, but it had no effect on the incubation period （P 〉0.05）.Compared with PB,combination of propofol and phenobarbital sodium ＋epileptic group had a lon-ger incubation period and a shorter plateau period with an increase of the expression of TLR4 protein in hippo-campus （P〈0.05 ）.Compared with propofol group, combination of propofol and phenobarbital sodium ＋ep-ileptic group had a shorter plateau period （P〈0.05） with an obvious increase in the expression of TLR4 pro-tein in hippocampus （P〈0.05）,but it had no effect on incubation period （P〉0.05 ）.Conclusions Propofol damages the learning and memorizing ability of epileptic rats.Phenobarbital sodium had no obvious effect on the learning ability in epileptic rats,but harms the memorizing ability in epileptic rats.Combi-nation of propofol and phenobarbital sodium affects

关 键 词：癫痫 丙泊酚 学习记忆 海马 苯巴比妥钠 水迷宫 

分 类 号：R332[医药卫生—人体生理学] R322.81[医药卫生—基础医学]