黄连生物碱促小鼠睡眠实验研究  

Study of Rhizoma Coptidis alkaloids on promoting sleep in mice

作　　者：邹宗尧 王燕枝[1] 胡慭然[1] 夏爽[1] 王德珍[1] 庞婕[1] 李学刚[1,2,3] 

机构地区：[1]西南大学药学院 [2]重庆市药效评价工程技术研究中心 [3]重庆市药物过程与质量控制工程技术研究中心,重庆400715

出　　处：《中国药理学通报》2014年第12期1752-1756,共5页Chinese Pharmacological Bulletin

基　　金：国家科技支撑计划资助项目(No 2011BAI13B02-1);西南大学校地合作基金资助项目(No Sz201302;Sz201401);重庆百名高端工程技术人才培养计划

摘　　要：目的研究5种黄连生物碱(小檗碱、黄连碱、巴马汀、表小檗碱、药根碱)对小鼠睡眠的影响,初步探讨其作用机制。方法通过小鼠自主活动实验和阈上剂量、阈下剂量戊巴比妥钠诱导小鼠睡眠实验,观察药物对小鼠入睡行为的影响。同时建立PCPA小鼠失眠模型,利用高效液相-荧光检测器,检测其下丘脑中神经递质NE、DA和5-HT的含量。结果与对照组相比,在行为学实验中ig 75 mg·kg-1·d-1的小檗碱和黄连碱能明显抑制小鼠的自主活动能力,且增加阈下剂量戊巴比妥钠小鼠入睡百分率。与模型组相比,小檗碱和黄连碱的NE和5-HT含量明显增加(P<0.01),DA含量无影响(P>0.05)。结论小檗碱和黄连碱可能通过增加PCPA失眠模型小鼠下丘脑中5-HT和NE含量,来发挥一定的镇静、催眠的作用,且小檗碱作用强于黄连碱。其余各生物碱对小鼠睡眠无影响。Aim To study the hypnotic effect of fivealkaloids extracted from Rhizoma Coptidis （ berberine ,coptisine, palmatine, epiberberine, jatrorrhizine）in mice, and preliminarily explore its underlying mecha- nism. Methods The experiments of locomotor activity and hypnosis induced by suprathreshold and subthresh- old doses of pentobarbital sodium were used to evaluate the effect of drugs on sleep behavior in mice. Then, HPLC-FLD was used to detect the contents of NE, DA and 5-HT on PCPA mice model. Results Compared with control group, berberine and coptisine notably in- hibited spontaneous activity in behavioral experiments （P 〈 0.05）, and increased the sleeping percentage of mice under subthreshold dose of pentobarbital sodium. Berberine and coptisine shortened the period of sleep latency, and prolonged the sustained period of sleeping at suprathreshold dose in mice （P 〈 0. 05 or P 〈0.01 ）. Other alkaloids had no significant differences in sleep latency and period of sleep observed in this current experiment. Compared with PCPA mice model group, berberine and coptisine remarkably increased the contents of NE and 5-ttT （P〈0.01 ）, but they had no effects on DA. Conclusions Berberine and coptisine may play a sedative and hypnotic role in PC- PA mice by increasing contents of 5-HT and NE in hy- pothalamus, and the sedative and hypnotic effects of berberine are stronger than those of coptisine. Other alkaloids have no effects on sleeping in mice.

关 键 词：黄连生物碱 神经递质 镇静 催眠 戊巴比妥钠 下丘脑-垂体-肾上腺素轴 

分 类 号：R332[医药卫生—人体生理学] R282.71[医药卫生—基础医学]