大鼠弥漫性轴索损伤后海马区caveolin-1的表达及其与星形胶质细胞活化的关系  

Changes of caveolin-1 expression in the hippocampus of rats after diffuse axonal injury and its relationship with activation of astroglia

作　　者：赵永林[1] 宋锦宁[1] 马旭东[1] 张斌飞[1] 张明[1] 李丹东[1] 庞宏刚[1] 罗显华[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2014年第6期747-752,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30471774);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);陕西省自然科学基金资助项目(No.2003C1-16)~~

摘　　要：目的研究大鼠弥漫性轴索损伤(diffuse axonal injury,DAI)后小窝蛋白1(caveolin-1)在大鼠脑海马区的表达,探讨caveolin-1的表达变化与星形胶质细胞活化的关系。方法运用Western blot检测大鼠海马中caveolin-1的表达,运用免疫组化检测海马区胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达,并通过免疫荧光共定位分析caveolin-1与GFAP之间的相互作用。结果 Western blot结果提示DAI后海马区caveolin-1的表达从1d开始显著降低,并持续降低至7d(P<0.05);免疫组化结果提示DAI后星形胶质细胞活化标志物GFAP的表达从1d后逐渐升高,3d后达峰,7d后稍降低,但仍高于正常(P<0.05);免疫荧光共定位提示DAI后caveolin-1与GFAP的共定位细胞数随着时间的延长逐渐增加。结论大鼠DAI后海马区caveolin-1的表达减少,GFAP的表达增加,星形胶质细胞内caveolin-1的表达变化可能是导致GFAP升高及星形胶质细胞活化的机制之一。Objective To explore the dynamic expression of cavcolin-1 in rat hippocampus after diffuse axonal injury （DAI） and its role in activation of astroglia. Methods Western blot was used to determine the expression of caveolin-1 in the hippocampus after DAI; immunohistochemistry staining was used to determine the dynamic expression of GFAP in the hippocampus of rats after DAI. The expression and co-localization of caveolin-1 and GFAP in rats after DAI were evaluated by immunofluorescence staining at different time points. Results The expression of caveolin-1 significantly decreased after DAI, and continued to decline from day 1 to day 7 （P^0.05）. Immunohistochemistry staining indicated that GFAP-positive cells significantly increased from day 1 to day 3, and slightly decreased at day 7 （P〈0.05）. The co-localization of caveolin-1 and GFAP revealed that the number of both caveolin-i- and GFAP-positive cells gradually increased. Conclusion Caveolin-1 expression significantly decreased with the increase of GFAP expression in the hippocampus of rats after DAI. Caveolin-1 may be involved in the dynamic expression of GFAP and play an important role in the activation of astroglia after DAI.

关 键 词：弥漫性轴索损伤 小窝蛋白1 星形胶质细胞活化 海马 大鼠 CAVEOLIN-1 

分 类 号：R651.1[医药卫生—外科学]