氢气通过抗氧化应激减轻大鼠弥漫性轴索损伤  

Attenuation of diffuse axonal injury by intraperitoneal injection of hydrogen via reducing oxidative stress in rats

作　　者：马旭东[1] 宋锦宁[1] 张斌飞[1] 赵永林[1] 孙鹏[1] 李丹东[1] 赵雅慧[1] 刘尊伟[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2014年第6期760-766,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30471774);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);陕西省自然科学基金资助项目(No.2003C1-16)~~

摘　　要：目的探讨氧化应激在大鼠弥漫性轴索损伤(DAI)中的作用及氢气对其治疗作用。方法将118只SD雄性大鼠随机平均分为假手术组、DAI模型组和氢气干预组。采用头颅瞬间旋转损伤装置制作大鼠DAI模型,干预组每日2次腹腔注射高纯度氢气(10mL/kg)。各组于预定时间点行神经功能评分、组织形态学、碘化丙啶(PI)染色及脑组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和丙二醛(MDA)含量检测。结果与假手术组比较,DAI后脑组织MDA含量升高(P<0.01),SOD及CAT活力下降(P<0.01),神经细胞肿胀,大量神经元变性、坏死,胶质细胞增多,PI阳性细胞增加(P<0.01),神经功能缺损加重(P<0.01)。给予氢气干预后脑组织MDA显著下降(P<0.01),SOD及CAT活力增强(P<0.01),组织形态学观察改善,PI阳性细胞数减少(P<0.01),神经功能缺损改善(P<0.05)。结论氧化应激参与了DAI急性期脑损伤的病理生理过程,自由基的大量产生及抗氧化酶活力的下降可能导致脑损伤的加重;氢气通过改善抗氧化酶活力并减轻自由基损伤改善DAI急性期脑损伤。减轻氧化应激程度对DAI急性期脑保护具有重要意义。Objective To investigate the role of therapeutic action of hydrogen in DAI models. Methods oxidative stress in diffuse axonal injury （DAI） and the Totally 118 adult male SD rats were divided into 3 groups with 39 in each： sham, DAI, and DAI＋ hydrogen treatment. Rat diffuse axonal injury was induced by an experimental facility, which was developed to make the rat head spin 90 degrees at the moment to cause shearing injury. Hydrogen gas of high purity was injected intraperitoneally into rats of DAI model twice per day （10 mL/kg）. The modified neurological severity score （mNSS）, histomorphology, propidium iodide （PI） staining, SOD activity, CAT activity and MDA content in the brain were assayed at 6 h, 1 d, 3 d and 7 d after DAI. Results MDA content, PI positive cells, swelling never cells, degenerated cells, glial cells and neurological severity scores were significantly increased, while SOD and CAT activities were decreased in the brain tissues of DAI rats （P〈0.01）. Hydrogen treatment could reverse these changes mentioned above. Conclusion Oxidative stress is involved in DAI pathophysiological process in acute phase. The decline of antioxidant enzyme activities and production of large numbers of free radicals may lead to the deterioration of brain damage. Hydrogen gas can improve acute brain injury in DAI by improving antioxidant enzyme activities and reducing free radical damage. Reducing the level of oxidative stress is of great significance in the treatment of DAI.

关 键 词：弥漫性轴索损伤 氧化应激 自由基 氢气 大鼠 

分 类 号：R651.1[医药卫生—外科学]