甲型H1N1流感病毒裂解疫苗在动物体内诱导中和抗体水平及其过敏原性  

Neutralizing antibody level induced and anaphylaxis of influenza A H1N1 virus split vaccine in animals

作　　者：赵大鹏[1] 李晓波[1] 郭雪[1] 任琦[1] 王亚军[1] 支百惠[1] 李晓辕[2] 吴业红[3] 曹宇[1] 商凤云[1] 张雪梅[1] 

机构地区：[1]长春生物制品研究所有限责任公司,吉林长春130062 [2]吉林大学第一医院呼吸科,吉林长春130021 [3]中国科学院长春应用化学研究所高分子物理与化学国家重点实验室,吉林长春130022

出　　处：《中国生物制品学杂志》2014年第10期1240-1242,共3页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(20090953)

摘　　要：目的评价甲型H1N1流感病毒裂解疫苗在动物体内诱导的中和抗体水平及其过敏原性。方法将昆明小鼠随机分为7组,分别经腹腔注射3批甲型H1N1流感病毒裂解疫苗(剂量分别为15、30、45μg/0.5 ml)、3批季节性H1N1流感病毒裂解疫苗(剂量分别为15、30、45μg/0.5 ml)和PBS(阴性对照),每只0.5 ml,间隔21 d加强免疫1次,分别于免疫前、初次免疫后21 d及加强免疫后14 d采血,分离血清,采用固定病毒-稀释血清法检测小鼠血清中和抗体滴度。将豚鼠随机分为4组,分别经腹腔注射上述3批甲型H1N1流感病毒裂解疫苗和PBS(阴性对照)各0.5 ml,隔日注射1次,共3次,第1次注射后第14和21 d,分别经静脉注射同一批号的疫苗1.0 ml攻击,观察豚鼠的过敏反应。结果初次免疫后21 d,甲型H1N1流感病毒裂解疫苗15、30、45μg剂量组免疫小鼠血清中和抗体滴度分别为免疫前和阴性对照组的47.9、113.5和319.9倍,且呈剂量依赖性;加强免疫后14 d,甲型H1N1流感病毒裂解疫苗各剂量组中和抗体滴度比初次免疫后21 d均明显升高,也呈剂量依赖性,且均高于与相应剂量的季节性H1N1流感病毒裂解疫苗。甲型H1N1流感病毒裂解疫苗各剂量组豚鼠均无过敏反应发生。结论甲型H1N1流感病毒裂解疫苗在昆明小鼠体内可诱导产生保护性中和抗体,对豚鼠无过敏反应。Objective To evaluate the neutralizing antibody level induced and anaphylaxis of influenza A H1N1 virus split vaccine in animals. Methods Kunming mice were divided randomly into seven groups,10 for each,and injected i.p.with three batches of influenza A H1N1 virus split vaccine at dosages of 15,30 and 45 μg / 0. 5 ml,three batches of seasonal influenza H1N1 virus split vaccine at dosages of 15,30 and 45 μg / 0. 5 ml and 0. 5 ml PBS（negative control）respectively,and boosted 21 d later. Serum samples were collected before immunization,21 d after primary immunization and 14 d after booster immunization respectively,and determined for neutralizing antibody titer by fixed virus-diluted serum method. Guinea pigs were divided randomly into four groups and injected i.p. with three batches of influenza A H1N1 virus split vaccine and PBS（negative control）respectively,0. 5 ml for each,every other day for 3 times,then challenged i.v. with 1. 0 ml of vaccine of the same batches on days 14 and 21 after the first immunization and observed for anaphylaxis. Results The neutralizing antibody titers in sera of mice 21 d after primary immunization with influenza A H1N1 virus split vaccine at dosages of 15,30 and 45 μg were 47. 9,113. 5 and 319. 9 times of those before immunization and those in negative control group,which were dose-dependent. However,the neutralizing antibody titers14 d after booster immunization with influenza A H1N1 virus split vaccine at various dosages were significantly higherthan those 21 d after primary immunization and those after booster immunization with seasonal influenza A virus split vaccine,which were also dose-dependent. No anaphylaxis was observed in guinea pigs immunized with influenza A H1N1 virus split vaccine at any dosages. Conclusion Influenza A H1N1 virus split vaccine induced protective neutralizing antibody, which showed no anaphylaxis in guinea pigs.

关 键 词：甲型H1N1流感病毒裂解疫苗 中和抗体 过敏原性 

分 类 号：R373.13[医药卫生—病原生物学] R392.33[医药卫生—基础医学]