趋化因子RANTES基因多态性和肺结核易感性的关系  

Research on the association of gene polymorphism of chemokine RANTES with tuberculosis susceptibility

作　　者：张建武[1] 邢志伟[1] 李玉静[1] 贾建雷[1] 翟倩[1] 

机构地区：[1]河北省胸科医院,河北石家庄050041

出　　处：《现代中西医结合杂志》2014年第33期3649-3651,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省医学科学研究重点课题计划项目(20120233)

摘　　要：目的研究RANTES基因启动子区-403G/A、-28C/G和内含子区Inl.1T/C的多态性和结核病易感性之间的关系。方法采用病例对照研究方法,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对78例结核病患者(病例组)和97例健康对照者(对照组)的RANTES-403G/A、-28C/G和Inl.1T/C多态性进行基因分型,分析这三个位点多态性与结核病发生率的关系。结果 2组RANTES-403G/A位点GG、GA和AA基因型的分布频率比较差异无统计学意义(x2=0.41,P>0.05),G、A等位基因分布频率差异无统计学意义(x2=0.30,P>0.05)。2组RANTES-28C/G位点CC、CG和GG基因型的分布频率比较差异无统计学意义(x2=0.15,P>0.05),C、G等位基因分布频率差异无统计学意义(x2=0.02,P>0.05)。2组RANTES Inl.1T/C位点TT、TC和CC基因型的分布频率比较差异无统计学意义(x2=0.53,P>0.05),T、C等位基因分布频率差异无统计学意义(x2=0.19,P>0.05)。结论 RANTES基因启动子区的-403G/A、-28C/G和内含子区的Inl.1T/C位点的单核苷酸多态性(SNP)与肺结核感染的易感性不相关。Objective It is to investigate the relationship of gene polymorphisms of RANTES promoters -403G/A, -28C/G and its intron Inl.1T/C with susceptibility to tuberculosis.Methods The gene polymorphisms of RANTES -403G/A, -28C/G and Inl.1T/C of 78 patients with tuberculosis （case group） and 97 healthy controls （control group ） were genotyped by polymerase chain reaction -restriction fragment length polymorphism （PCR -RFLP） in the case -control study.The correla-tion between gene polymorphism of them and susceptibility to tuberculosis were analyzed.Results There was no significant difference in GG, GA and AA genotype frequency of RANTES -403G/A between the case group and the control group （χ^2 =0.41, P 〉0.05）; and no significant difference in C, G allele frequency distribution between both groups （χ^2 =0.02, P 〉0. 05）.There was no significant difference in CC, CG and GG genotype frequency of RANTES -28C/G between both groups （χ^2 =0.15, P 〉0.05）; and no significant difference in C, G allele frequency distribution between both groups （χ^2 =0.02, P 〉0.05）.There was no significant difference in TT, TC and CC genotype frequency of RANTES Inl.1T/C between both groups （χ^2 =0.53, P 〉0.05）; and no significant difference in T, C allele frequency distribution between both groups （χ^2 =0.19, P 〉0.05）.Conclusion Single nucleotide polymorphism （SNP） of RANTES promoters -403G/A, -28C/G and its intron Inl.1T/C may not be related to the susceptibility to tuberculosis infection.

关 键 词：趋化因子 RANTES 基因多态性 肺结核 

分 类 号：R521.9[医药卫生—内科学]